Prazosin

Manufactured by Abcam

Sourced in United Kingdom

Prazosin is a selective alpha-1 adrenergic receptor antagonist. It is used as a laboratory tool to study the pharmacology and physiology of the alpha-1 adrenergic receptor.

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Lab products found in correlation

Prazosin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Thapsigargin, by Bio-Techne (1 mentions) Isradipine, by Alomone (1 mentions) Bafilomycin, by Bio-Techne (1 mentions) Noradrenaline, by Bio-Techne (1 mentions) Ned 19, by Bio-Techne (1 mentions) Xestospongin c, by Bio-Techne (1 mentions) Esi 05, by Biolog (1 mentions) ω agatoxin, by Alomone (1 mentions)

2 protocols using prazosin

Prazosin and Inhibitor Effects on EPCs

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EPCs were isolated from the middle cerebral artery in experimental rats (the sham group) and cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) medium with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc.) and incubated overnight at 37˚C in a humidified atmosphere of 5% CO₂. EPCs were treated with 5 mg/ml prazosin (Sigma-Aldrich; Merck KGaA) and/or Akt inhibitor (AktIR; 1 mg/ml; cat. no. ab14088; Abcam) and/or NF-κB inhibitor (NF-κBIR; 1 mg/ml; cat. no. ab141588; Abcam) for 24 h at 37˚C to analyze the therapeutic effects of prazosin.

Liu S, & Li W. (2020). Prazosin blocks apoptosis of endothelial progenitor cells through downregulating the Akt/NF-κB signaling pathway in a rat cerebral infarction model. Experimental and Therapeutic Medicine, 20(3), 2577-2584.

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Modulation of Ca2+ Signaling Pathways

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The following substances were used (source given in parentheses): the L-type Ca²⁺ channel blocker isradipine and the P/Q Ca²⁺ channel blocker ω-agatoxin (Alomone Labs; Jerusalem, Israel); the α₁-antagonist prazosin (Abcam, Cambridge, UK); the membrane-permeable PKA inhibitor myr-PKI, the IP₃ receptor inhibitor Xestospongin C, the NAADP antagonist Ned-19, the V-ATPase inhibitor bafilomycin, the sER ATPase inhibitor thapsigargin and noradrenaline (Tocris Bioscience, Bristol, UK); the EPAC2 inhibitor ESI-05 (BioLog, Bremen, Germany); the insulin receptor antagonist (S961, Novo-Nordisk, Denmark). All other compounds were obtained from Sigma-Aldrich (Dorset, UK).

Hamilton A., Zhang Q., Salehi A., Willems M., Knudsen J.G., Stephen S., Ringgaard A.K., Chapman C.E., Gonzalez-Alvarez A., Surdo N.C., Zaccolo M., Basco D., Johnson P.R., Ramracheya R., Rutter G.A., Galione A., Rorsman P, & Tarasov A.I. (2018). Adrenaline stimulates glucagon secretion by Tpc2-dependent Ca2+ mobilization from acidic stores in pancreatic α-cells. Diabetes, 67(6), 1128-1139.

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