Cxcl12tm2.1sjm j

Manufactured by Jackson ImmunoResearch

Cxcl12tm2.1Sjm/J is a targeted mutation mouse model that contains a knock-in of the mouse Cxcl12 gene with a mutation at the second codon. This model can be used to study the role of the CXCL12 protein in various biological processes.

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2 protocols using cxcl12tm2.1sjm j

Genetic Manipulation of Murine Vascular Biology

Cited 2 times

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All mouse colonies were housed and bred in the animal facility at Stanford University in accordance with institutional animal care and use committee (IACUC) guidance. All mouse lines were described earlier: Cx40CreER (Miquerol et al., 2015 (link)), ApjCreER (Chen et al., 2014 (link)), Tie2::eGFP::Claudin5 (Knowland et al., 2014 (link); Lutz et al., 2017 (link)). Cdh5CreER transgenic mice (Wang et al., 2010 (link)). Cxcl12^fl/fl (The Jackson Laboratory, B6(FVB)-Cxcl12^tm1.1Link/J, Stock# 021773), Cxcr4^fl/fl (The Jackson Laboratory, B6.129P2-Cxcr4^tm2Yzo/J, Stock# 008767), Rosa26^TdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-TdTomato)Hze}/J, Stock# 007909) Rosa26^Zsgreen (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm6(CAG-zsGreen1)Hze}/J, Stock# 007906), and Cxcl12-DsRed (The Jackson Laboratory, Cxcl12^tm2.1Sjm/J, Stock# 022458). For neonatal studies, all Tamoxifen injections to activate Cre activity (6mgs in corn oil) were given intraperitoneally to nursing mothers. Due to mouse genetic inheritance, analyses were compiled from age-matched males and females from multiple litters.

Das S., Goldstone A.B., Wang H., Farry J., D’Amato G., Paulsen M.J., Eskandari A., Hironaka C.E., Phansalkar R., Sharma B., Rhee S., Shamskhou E.A., Agalliu D., de Jesus Perez V., Woo Y.J, & Red-Horse K. (2019). A unique collateral artery development program promotes neonatal heart regeneration. Cell, 176(5), 1128-1142.e18.

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Genotyping and Analysis of CXCL12-Deficient Mice

Cited 1 time

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Development and genotyping information of mouse lines Cxcl12^tm2.1Sjm/J (The Jackson Laboratory, stock #022458, Cxcl12^DsRed) and Cxcl12^tm1.1Sjm/J (The Jackson Laboratory, stock #022457, Cxcl12^loxp/loxp) have been previously described (Ding and Morrison, 2013 (link)). The endothelial-specific Cre driver Tek^Cre [Tg(Tek-Cre^1Ywa), stock #008863] was used to delete CXCL12 in the endothelium (Kisanuki et al., 2001 (link)). Timed matings were performed, counting the day of plug as E0.5. Lungs were harvested from embryos from both heterozygous (Cxcl12^DsRed/+) and homozygous knockout (Cxcl12^Dsred/DsRed) mice, and they were prepared as noted below for whole-mount and tissue section staining. All animal studies were performed in adherence to the guidelines of the Children's Hospital of Philadelphia Institutional Animal Care and Use Committee.

Chandrasekaran P., Negretti N.M., Sivakumar A., Liberti D.C., Wen H., Peers de Nieuwburgh M., Wang J.Y., Michki N.S., Chaudhry F.N., Kaur S., Lu M., Jin A., Zepp J.A., Young L.R., Sucre J.M, & Frank D.B. (2022). CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth. Development (Cambridge, England), 149(21), dev200909.

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