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Cary 5000 uv vis nir absorption spectrophotometer

Manufactured by Agilent Technologies
Sourced in United States

The Cary 5000 UV-VIS-NIR absorption spectrophotometer is a versatile instrument designed for measuring the absorption of light by a sample across a wide range of the electromagnetic spectrum, from ultraviolet to near-infrared. It is capable of performing precise and accurate measurements of sample absorbance, transmittance, and reflectance.

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2 protocols using cary 5000 uv vis nir absorption spectrophotometer

1

Characterization of Nanocarrier Physicochemical Properties

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The size and the zeta potential of the NCs were measured using Zetasizer Nanoseries (Malvern Instruments, Malvern, Worcestershire, UK). All the measurements were performed 3 times at 25 °C. The fluorescence (Excitation: 405 nm, Emission: 650–750 nm) from VP in the NCs was measured using FluoroMax plus C (JY Horiba, Kyoto, Japan) by using a quartz cuvette at room temperature. To confirm and quantify the amount of VP, the absorption spectra of the NCs was analyzed by Cary 5000 UV-VIS-NIR absorption spectrophotometer (Varian Inc., Palo Alto, California, USA) using paired 1-cm pathlength cuvettes.
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2

Solvent Exchange for NC Optical Analysis

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The copper and
tin based NCs dispersed in toluene were dried under nitrogen flux
to remove the solvent and were redispersed in anhydrous TCE to perform
the measurements. The UV–vis–NIR absorption spectra
of the NCs solutions were recorded using a Varian Cary 5000 UV–vis–NIR
absorption spectrophotometer.
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