Secondary antibody conjugated to rhodamine red x

Chrysin, 2,4-dinitrochlorobenzene (DNCB), toluidine blue (TB), and hematoxylin and eosin (H&E) staining kits were obtained from Sigma-Aldrich (St. Louis, MO, USA). Low-melting agarose was purchased from Lonza (Rockland, ME, USA). CCL5 was obtained from PeproTech (London, UK). TNFα was obtained from ProSpec-Tany TechnoGene, Ltd. (Ness-Ziona, Israel). A Firefly Luciferase Assay System was obtained from Promega (Madison, WI, USA). Anti-CCL5 antibody was obtained from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA), and anti-IKKα/β, -IκB, -phospho-IκB (Ser32), -p65/RelA, and -phospho-p65/RelA (Ser536) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Secondary antibody conjugated to rhodamine red-X was obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Chrysin, DNCB, TB, and H&E staining kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). TNFα was purchased from ProSpec-Tany TechnoGene, Ltd. (Ness-Ziona, Israel). A firefly luciferase assay system was obtained from Promega (Madison, WI, USA). Anti-TSLP antibody was obtained from Novus Biologicals (Centennial, CO, USA), and phospho-ERK1/2 (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), and phospho-JNK1/2 (Thr183/Tyr185) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH and anti-EGR1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). A secondary antibody conjugated to rhodamine red-X was obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).