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Phospho mtorser2448

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Phospho-mTORSer2448 is a laboratory reagent that specifically detects phosphorylation of the mammalian target of rapamycin (mTOR) protein at the serine 2448 residue. This phosphorylation event is associated with the activation of the mTOR signaling pathway, which plays a key role in regulating cellular processes such as cell growth, proliferation, and metabolism.

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Lab products found in correlation

Hsp60, by Santa Cruz Biotechnology (2 mentions) Caspase 3, by Novus Biologicals (2 mentions) Ndp52, by BD (2 mentions) Phospho aktser473, by Novus Biologicals (2 mentions) Phospho p70 s6 kinasethr389, by Novus Biologicals (2 mentions) Phospho ulkser757, by Novus Biologicals (2 mentions) Tax1bp1, by Novus Biologicals (2 mentions) Optineurin, by Thermo Fisher Scientific (2 mentions) Caspase 9, by Novus Biologicals (2 mentions) Beclin 1, by Cell Signaling Technology (2 mentions) Pink1, by Proteintech (2 mentions) Image lab analysis software, by Bio-Rad (2 mentions) Gapdh, by Novus Biologicals (2 mentions) Chemidoc molecular imager, by Bio-Rad (2 mentions) Criterion tgx gel, by Bio-Rad (2 mentions) Trans blot turbo system, by Bio-Rad (2 mentions)

2 protocols using phospho mtorser2448

1

Immunoblotting of Autophagic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 15 μg of protein was loaded in precast 4–20% Criterion TGX gels (Bio-Rad Laboratories, Hercules, CA), transferred to a PVDF membrane with the Trans-BlotⓇ Turbo™ system (Bio-Rad Laboratories), blocked, incubated with primary antibodies, and incubated with the secondary antibody. Images were captured via the ChemiDoc Molecular Imager and bands were quantified with ImageLab analysis software (both from Bio-Rad). Expression levels were determined by chemiluminescent immunodetection and normalized to appropriate loading controls. Immunoblotting was performed using commercially available antibodies from Abcam (Cambridge, MA): NDP52 (1:500); BD Biosciences (San Jose, CA): P62 (1:1000); Cell Signaling Technology (Danvers, MA): Beclin-1 (1:750), Caspase 3 (1:500), Caspase 9 (1:1000), GAPDH (1:40,000), LC3 (1:1000), mTOR (1:1000), NBR1 (1:500), phospho-AKTSer473 (1:1000), phospho-mTORSer2448 (1:500), phospho-P70 S6 KinaseThr389 (1:1000), phospho-ULKSer757 (1:1000), RAB7 (1:1000), and TAX1BP1 (1:1000); Novus Biologicals (Littleton, CO): PINK1 (1:250); Proteintech Group, Inc. (Rosemont, IL): Optineurin (1:500); Thermofisher Scientific (Waltham, MA): TFEB (1:500); and Santa Cruz Biotechnology (Dallas, TX): HSP60 (1:5000).
Cheng G., Hardy M., Zielonka J., Weh K., Zielonka M., Boyle K.A., Abu Eid M., McAllister D., Bennett B., Kresty L.A., Dwinell M.B, & Kalyanaraman B. (2020). Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer treatment and research communications, 25, 100210.
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2

Immunoblotting of Autophagic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 15 μg of protein was loaded in precast 4–20% Criterion TGX gels (Bio-Rad Laboratories, Hercules, CA), transferred to a PVDF membrane with the Trans-BlotⓇ Turbo™ system (Bio-Rad Laboratories), blocked, incubated with primary antibodies, and incubated with the secondary antibody. Images were captured via the ChemiDoc Molecular Imager and bands were quantified with ImageLab analysis software (both from Bio-Rad). Expression levels were determined by chemiluminescent immunodetection and normalized to appropriate loading controls. Immunoblotting was performed using commercially available antibodies from Abcam (Cambridge, MA): NDP52 (1:500); BD Biosciences (San Jose, CA): P62 (1:1000); Cell Signaling Technology (Danvers, MA): Beclin-1 (1:750), Caspase 3 (1:500), Caspase 9 (1:1000), GAPDH (1:40,000), LC3 (1:1000), mTOR (1:1000), NBR1 (1:500), phospho-AKTSer473 (1:1000), phospho-mTORSer2448 (1:500), phospho-P70 S6 KinaseThr389 (1:1000), phospho-ULKSer757 (1:1000), RAB7 (1:1000), and TAX1BP1 (1:1000); Novus Biologicals (Littleton, CO): PINK1 (1:250); Proteintech Group, Inc. (Rosemont, IL): Optineurin (1:500); Thermofisher Scientific (Waltham, MA): TFEB (1:500); and Santa Cruz Biotechnology (Dallas, TX): HSP60 (1:5000).
Cheng G., Hardy M., Zielonka J., Weh K., Zielonka M., Boyle K.A., Abu Eid M., McAllister D., Bennett B., Kresty L.A., Dwinell M.B, & Kalyanaraman B. (2020). Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer treatment and research communications, 25, 100210.
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