Pd minitrap g 25 size exclusion column

Manufactured by GE Healthcare

The PD MiniTrap G-25 is a size exclusion column designed for desalting and buffer exchange. It is used to separate small molecules from larger molecules in a sample.

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Lab products found in correlation

Q700 sonicator, by Qsonica (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Pd mini trap, by Cytiva (1 mentions)

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G-25 column (60 citations) PD MiniTrap G-25 (35 citations)

2 protocols using pd minitrap g 25 size exclusion column

Bacterial and Human Cell Protein Extraction

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Overnight cultures of P. aeruginosa or B. subtilis (grown in 5 mL 2XYT media at 37 °C) and human Jurkat cells (grown in RPMI-1640 Medium with 10% heat-inactivated fetal bovine serum) were divided into 100-mg wet pellets. The cells were suspended in 500 μL PBS, pH 7.4 and sonicated in a Qsonica Q700 sonicator with cup horn attachment at 25% amplitude for 10 min at 4 °C. Insoluble cellular debris was removed by centrifugation at 10 000g for 5 min at room temperature, and the supernatant was carefully removed and filtered through a PD MiniTrap G-25 size exclusion column (GE Life Sciences, No. 28918007) to remove residual, low molecular-weight contaminants. The soluble protein content in the flow-through (samples designated as A-supernatant, see Supporting Information, Figure 1 for details) was then quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher, No. 23225).

Wang A.Y., Thuy-Boun P.S., Stupp G.S., Su A.I, & Wolan D.W. (2018). Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass. Journal of proteome research, 17(9), 2978-2986.

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Radiolabeling DOXIL Liposomes with [52Mn]Mn

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[⁵²Mn]Mn(oxinate)₂ (150 μL, 3.2% v/v DMSO in water) was added to a solution of DOXIL (350 μL, 2 mg mL^–1) and the mixture heated with frequent agitation at 50 °C for 30 min. The mixture was then loaded onto a PD minitrap G-25 size exclusion column (GE Healthcare) following the manufacturers’ gravity protocol. The labelled liposomes ([⁵²Mn]Mn-DOXIL) were then obtained by adding saline (750 μL) to the column and collecting the eluate.

Gawne P., Man F., Fonslet J., Radia R., Bordoloi J., Cleveland M., Jimenez-Royo P., Gabizon A., Blower P.J., Long N, & de Rosales R.T. (2018). Manganese-52: applications in cell radiolabelling and liposomal nanomedicine PET imaging using oxine (8-hydroxyquinoline) as an ionophore †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8dt00100f. The data supporting this research are openly available from the King's College London research data archive at http://dx.doi.org/10.18742/RDM01-283.. Dalton Transactions (Cambridge, England : 2003), 47(28), 9283-9293.

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