Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum and 0.25% Trypsin-EDTA were purchased from Gibco Life TechnologiesTM. The 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Sigma–Aldrich Co. Anti-rabbit horseradish peroxidase-conjugated secondary antibody, anti-mouse horseradish peroxidase-conjugated secondary antibody, anti-β-actin antibody, anti-Cytochrome P2E1 antibody, and anti-CYP3A4 antibody were obtained from Abcam. Anti-CYP1A2 rabbit polyclonal antibody was purchased from Sangon Biotech (Shanghai) Co., Ltd. PierceTM BCA Protein Assay Kit and RevertAid First Strand cDNA Synthesis Kit were purchased from Thermo Fisher Scientific. All-in-OneTM qPCR Mix Kit was purchased from GeneCopoeia. TRIzoL Reagent was bought from Invitrogen. Western blotting luminal reagent was obtained from Santa Cruz Biotechnology, Inc. Acetonitrile, methanol, formic acid, and reference substances (caffeine, chlorzoxazone, dapsone, naringenin, hesperetin, and meranzin hydrate) were HPLC grade, other reagents were analytical grade.
Anti cyp3a4 antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-CYP3A4 antibody is a laboratory tool used for the identification and detection of the CYP3A4 protein. CYP3A4 is a key enzyme involved in the metabolism of a wide range of drugs and other xenobiotics. This antibody can be used in various analytical techniques, such as Western blotting and immunohistochemistry, to study the expression and localization of CYP3A4 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (3 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Caffeine, by Santa Cruz Biotechnology (1 mentions)
Naringenin, by Santa Cruz Biotechnology (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Horseradish peroxidase conjugated anti rabbit secondary antibody, by Abcam (1 mentions)
Western blotting luminal reagent, by Santa Cruz Biotechnology (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
Nadph regenerating system, by Promega (1 mentions)
Dextrose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Supersignal west pico trial kit, by Thermo Fisher Scientific (1 mentions)
Taq plus master mix, by Vazyme (1 mentions)
Mouse monoclonal anti or antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti cyp3a4 antibody
1
DMEM-based Cell Culture Protocol
2
Yeast-based CYP3A4 Activity Assay
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The FinePure Universal DNA Purification Kit was supplied by GENFINE Biotech in Beijing, China. Taq plus master mix was purchased from Vazyme (Nanjing, China). Restriction enzymes, along with DO Supplement-Ura, all purchased from Takara Bio, Inc (Otsu, Shiga, Japan). Yeast strain YPH499 was obtained from the American Type Culture Collection (ATCC) in Virginia, USA. The growth mediums for yeast, specifically the yeast nitrogen base devoid of amino acids, along with dextrose, galactose, and MDZ, were supplied by Sigma-Aldrich (Missouri, USA). The rabbit polyclonal anti-CYP3A4 antibody was obtained from Abcam (Cambridge, UK) and the mouse monoclonal anti-OR antibody was from Santa Cruz Biotechnology in Dallas, Texas, USA.
The chemical 1-hydroxy Midazolam (1'-OH-MDZ) was obtained from Toronto Research Chemicals, Inc. (Toronto, Ontario, Canada). The NADPH-regenerating system was purchased from Promega in Madison, Wisconsin, USA. The Super Signal West Pico Trial Kit and highpressure liquid chromatography-grade solvents were acquired from Thermo Fisher Scientific Co.
in Waltham, Massachusetts, USA. All other chemicals and solvents used in our experiments were of the highest purity and analytical grade available on the market.
The chemical 1-hydroxy Midazolam (1'-OH-MDZ) was obtained from Toronto Research Chemicals, Inc. (Toronto, Ontario, Canada). The NADPH-regenerating system was purchased from Promega in Madison, Wisconsin, USA. The Super Signal West Pico Trial Kit and highpressure liquid chromatography-grade solvents were acquired from Thermo Fisher Scientific Co.
in Waltham, Massachusetts, USA. All other chemicals and solvents used in our experiments were of the highest purity and analytical grade available on the market.
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