Dxh 600 hematology analyzer

On the days of blood collection, all 3 mL EDTA tubes were refrigerated at 4 °C. Following all testing for the day, tubes were transported to the CLIA-certified Auburn University Medical Clinic, and complete blood count (CBC) panels were analyzed using Beckman-Coulter DxH 600 Hematology analyzer (Beckman Coulter, Fullerton, CA, USA). Specifically, the following whole blood parameters were determined: total white blood cells (WBCs), neutrophil differentials (absolute counts and percentage of WBCs), lymphocyte differentials (absolute counts and percentage of WBCs), and monocyte differentials (absolute counts and percentage of WBCs).
On the days of blood collection, serum was also obtained from 5 ml serum collection tubes through centrifugation at 3500 x g for 5 min at room temperature. Serum aliquots were then placed in 1.7 ml microcentrifuge tubes and stored at -20 °C until batch-processing for serum myoglobin. A human ELISA for myoglobin was used to determine serum concentrations (Abcam, Cambridge, MA, USA). Of note, it has been shown that increases in serum myoglobin concentration is a valid marker of muscle-damage as well as being more sensitive and less variable than creatine kinase [21 (link)].

Ten biochemical and six hematological parameters were measured in the serum of each patient selected from 17 military hospitals in Da Nang, Vietnam. An amount of 2 mL of venous blood was withdrawn from each patient for biochemical testing. Liver enzymes, consisting of GGT, AST and ALT, and other biochemical markers, including protein, cholesterol, triglyceride, total bilirubin, glucose, urea and creatinine, in the serum were measured using the fully automated Hitachi 912 Chemistry Analyzer (Roche Diagnostics, Mannheim, Germany). All reagent kits were obtained from Roche Diagnostics, India. Similarly, 2 mL of venous blood was used to measure the six hematological indices, including red blood cell count, hemoglobin, leukocytes, neutrocytes, lymphocytes and platelets, using the DxH 600 Hematology Analyzer (Beckman coulter, Seattle, WC, USA).

Peripheral blood samples were collected from participants and sent to the central laboratory of Vinmec Central Park Hospital. Vitamin B12 and acid folic levels were measured using a UniCel DxI 800 Access Immunoassay System (Beckman Coulter, CA, USA). Total blood count was obtained using a DxH 600 Hematology Analyzer (Beckman Coulter), and fasting plasma glucose, creatinine, AST, ALT, and lipid profile were measured using an AU680 Clinical Chemistry Analyzer (Beckman Coulter). HbA1c was measured by a Premier Hb9210 HbA1c Analyzer (Trinity Biotech, Ireland). Vitamin B12 deficiency was defined as a vitamin B12 level < 300pg/ml with a normal level of folic acid (≥ 4ng/ml).