Small interfering RNAs against IL-11 (sc-39636) or STAT3 (sc-29493) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant human IL-11 and IL-11 neutralizing antibody were purchased from R&D Systems (Minneapolis, MN, USA). Recombinant human IL-11 was reconstituted in PBS containing 0.5% bovine serum albumin (BSA) before being used in experiments.
Recombinant human il 11
Manufactured by R&D Systems
Sourced in United States
Recombinant human Interleukin-11 (IL-11) is a cytokine produced by a variety of cell types, including fibroblasts, stromal cells, and T cells. It is involved in the regulation of various biological processes, including hematopoiesis, inflammation, and immune response.
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Lab products found in correlation
2 protocols using recombinant human il 11
1
Targeting IL-11 and STAT3 in Research
2
Real-Time Monitoring of Uterine Cancer Cell Proliferation
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Experiments were carried out using the RTCA DP xCELLigence instrument (Roche), which was placed in a humidified incubator maintained at 37°C with 95% air/5% CO2. Cells were seeded in E-plate 96 at 10,000 cells/well in 5% FCS medium and the plate was monitored once every 15 min for a total of 72 h. AN3CA cells transfected with miR-1 mimic or scr control for 72 h were treated with/without recombinant human IL11 (100 ng/ml) (R&D systems) [20 ] at the time of seeding for xCELLigence real time proliferation assays. In subsequent experiments, AN3CA cells were treated with IgG control, or IL11Rα Ab (1 μg/ml), doxorubicin (500 ng/ml), or Ab and doxorubicin. Data was calculated using RTCA software 1.2, supplied with the instrument (ACEA) and exported for statistical analysis.
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