Lecithin (Sinopharm Chemical Reagent Ltd. Co., Shanghai, China), cholesterol (KeLong Chemical Reagent Ltd. Co., Chengdu, China) and PTX (Chengdu Yuancheng Biotechnology Ltd. Co., Chengdu, China), all in ethyl ether (3 : 1 : 0.3, weight ratio), were added to the reaction bottle. The mixture was kept in water bath at 55 °C by a rotary evaporator until the ethyl ether was completely evaporated. Then a given amount of PBS, PEG 4000, dextran 40000 and span 80 was added and mixed. After that, the air was displaced with the nitrogen gas. After being vibrated and centrifuged at 300 rpm for three minutes, 125 μl of 3% human serum albumin was added to the resulting PLM. For the control lipid MBs (LM), the same procedure was performed instead of PTX addition.
Cholesterol
Manufactured by Chron Chemicals
Sourced in China
The Cholesterol lab equipment is a device used to measure the concentration of cholesterol in a given sample. It provides an accurate and reliable analysis of cholesterol levels, which is a crucial biomarker for various health conditions.
Automatically generated - may contain errors
Lab products found in correlation
Lecithin, by Sinopharm (1 mentions)
Calf thymus dna, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein cfpe, by Avanti Polar Lipids (1 mentions)
Dspe peg 2000 ome, by Avanti Polar Lipids (1 mentions)
Scrambled sirna, by GenePharma (1 mentions)
Bcl 2 sirna, by GenePharma (1 mentions)
Cy5 labeled sirna, by GenePharma (1 mentions)
2 protocols using cholesterol
1
Preparation of Paclitaxel-loaded Lipid Microballoons
2
Lipid-Based Nanoparticle Delivery System for siRNA Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cholesterol was purchased from Chengdu Kelong Chemical Company (Chengdu, China). Soybean phospholipids (SPC) were purchased from Shanghai Taiwei Chemical Company (Shanghai, China). 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP) was obtained from Shanghai Advanced Vehicle Technology (AVT) Ltd. (Shanghai, China). DSPE-PEG2000-OMe and 1, 2-dioleoyl-sn-glycero-3- phosp-hoethanolamine-N-(carboxyfluorescein) (CFPE ) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Protamine sulfate (fraction X from salmon) and calf thymus DNA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paclitaxel (PTX) and low-molecular-weight heparin (Enoxaparin sodium, LH) were purchased from Melonepharma (Dalian, China). BCL-2 siRNA, scrambled siRNA and Cy5-labeled siRNA were purchased from Gene Pharma Ltd. (Shanghai, China). The BCL-2 siRNA (siBCL-2) sense sequence is 5'-GUG AUG AAG UAC AUC CAU UdTdT-3', and the anti-sense sequence is 5'-AAU GGA UGU ACU UCA UCA CdTdT-3'. The scramble siRNA (siCTL) sense sequence is 5'-UUC UCC GAA CGU GUC ACG UTT-3', and the anti-sense sequence is 5'-ACG UGA CAC GUU CGG AGA ATT-3'.
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