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Turner biosystems luminometer reader

Manufactured by Promega
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The Turner BioSystems Luminometer Reader is a compact and versatile instrument designed for luminescence-based assays. It measures light output from samples, providing quantitative data for a range of biological applications.

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Lab products found in correlation

E1910, by Promega (4 mentions)

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Turner Biosystems luminometer (8 citations)

4 protocols using turner biosystems luminometer reader

1

Luciferase Reporter Assay in HEK293T

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UAS-luciferase reporter plasmid, cytomegalovirus (CMV)–Renilla control plasmid, human DNA-modifying enzymes fusing with VP16 transactivation domain, and CEBPA fusing with Gal4-DBD were cotransfected into HEK293T cells. After transfection for 30 hours, the luciferase reporter activity was detected using a commercial kit (Promega, E1910) with a Turner BioSystems luminometer reader (Promega). The individual luciferase raw data are shown in table S3.
Chen X., Zhou W., Song R.H., Liu S., Wang S., Chen Y., Gao C., He C., Xiao J., Zhang L., Wang T., Liu P., Duan K., Cheng Z., Zhang C., Zhang J., Sun Y., Jackson F., Lan F., Liu Y., Xu Y., Wong J.J., Wang P., Yang H., Xiong Y., Chen T., Li Y, & Ye D. (2022). Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity. Science Advances, 8(4), eabl5220.
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2

High-throughput Transactivation Assay for TET2

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Human full-length TET2 fused to VP16 transactivation domain (AD), Preys (1,126 human ORFs) fused to Gal4 DBD, UAS-Luciferase reporter plasmid, and CMV-Renilla control plasmid were co-transfected in HEK293T cells. At 30 hours after transfection, the luciferase reporter activity was measured by a commercial kit (Promega E1910) using a Turner BioSystems Luminometer Reader (Promega).
When the AD-TET2 exists, the luciferase value is labeled as L1, and the Renilla value is labeled as R1. When the AD-TET2 does not exist, the luciferase value is labeled as L2, and the Renilla value is labeled as R2. The blank luciferase background is low and thus can be ignored. The relative luciferase activation is calculated as following: (L1/R1) / (L2/R2).
Chen L.L., Lin H.P., Zhou W.J., He C.X., Zhang Z.Y., Cheng Z.L., Song J.B., Liu P., Chen X.Y., Xia Y.K., Chen X.F., Sun R.Q., Zhang J.Y., Sun Y.P., Song L., Liu B.J., Du R.K., Ding C., Lan F., Huang S.L., Zhou F., Liu S., Xiong Y., Ye D, & Guan K.L. (2018). SNIP1 Recruits TET2 to Regulate c-MYC Target Genes and Cellular DNA Damage Response. Cell reports, 25(6), 1485-1500.e4.
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3

High-throughput Transactivation Assay for TET2

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Human full-length TET2 fused to VP16 transactivation domain (AD), Preys (1,126 human ORFs) fused to Gal4 DBD, UAS-Luciferase reporter plasmid, and CMV-Renilla control plasmid were co-transfected in HEK293T cells. At 30 hours after transfection, the luciferase reporter activity was measured by a commercial kit (Promega E1910) using a Turner BioSystems Luminometer Reader (Promega).
When the AD-TET2 exists, the luciferase value is labeled as L1, and the Renilla value is labeled as R1. When the AD-TET2 does not exist, the luciferase value is labeled as L2, and the Renilla value is labeled as R2. The blank luciferase background is low and thus can be ignored. The relative luciferase activation is calculated as following: (L1/R1) / (L2/R2).
Chen L.L., Lin H.P., Zhou W.J., He C.X., Zhang Z.Y., Cheng Z.L., Song J.B., Liu P., Chen X.Y., Xia Y.K., Chen X.F., Sun R.Q., Zhang J.Y., Sun Y.P., Song L., Liu B.J., Du R.K., Ding C., Lan F., Huang S.L., Zhou F., Liu S., Xiong Y., Ye D, & Guan K.L. (2018). SNIP1 Recruits TET2 to Regulate c-MYC Target Genes and Cellular DNA Damage Response. Cell reports, 25(6), 1485-1500.e4.
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4

ASXL1-VP16 Transactivation Assay

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Human full-length ASXL1 CDS fused to VP16 transactivation domain (AD), Preys fused to Gal4 DNA-binding domain (DBD), UAS-Luciferase reporter plasmid, and CMV-Renilla control plasmid were co-transfected in HEK293T cells. At 48 h after transfection, the luciferase reporter activity was measured by a commercial kit (Promega E1910) using a Turner BioSystems Luminometer Reader (Promega). When the ASXL1-VP16 (AD) exists, the luciferase value is labeled as L1, and the Renilla value is labeled as R1. When the ASXL1-VP16 (AD) does not exist, the luciferase value is labeled as L2, and the Renilla value is labeled as R2. The relative luciferase activation is calculated as following: (L1/R1)/(L2/R2).
Xia Y.K., Zeng Y.R., Zhang M.L., Liu P., Liu F., Zhang H., He C.X., Sun Y.P., Zhang J.Y., Zhang C., Song L., Ding C., Tang Y.J., Yang Z., Yang C., Wang P., Guan K.L., Xiong Y, & Ye D. (2020). Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network. Protein & Cell, 12(7), 557-577.
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