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E2 bsa fitc

Manufactured by Merck Group
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E2-BSA-FITC is a fluorescently labeled compound consisting of estradiol (E2) conjugated to bovine serum albumin (BSA) and labeled with fluorescein isothiocyanate (FITC). This compound is commonly used as a tool in research applications.

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Lab products found in correlation

Soluble 17β estradiol e2, by Merck Group (2 mentions) Extracellular rabbit anti maxik α, by Alomone (2 mentions) Extracellular rabbit anti maxik β1, by Novus Biologicals (2 mentions) Methyl β cyclodextrin mβcd, by Merck Group (1 mentions)

Close spelling variants of this product

E2-BSA (4 citations) FITC-BSA (83 citations)

2 protocols using e2 bsa fitc

1

Fluorescent Labeling of Ion Channels

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Membrane impermeable E2-BSA-FITC and soluble 17β-Estradiol (E2) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Extracellular rabbit anti-MaxiK-α was purchased from Alomone Labs (Jerusalem, Israel), Intracellular goat anti-KCa1.1 antibody from Santa Cruz Biotechnology (Dallas, TX, USA) and extracellular rabbit anti-MaxiK-β1 from Novus Biologicals (Littleton, CO, USA). Nuclear marker 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) and secondary antibodies anti-rabbit Alexa Fluor 488, 568 and anti-goat Alexa Fluor 488 were acquired from Invitrogen (Waltham, MA, USA).
Granados S.T., Castillo K., Bravo-Moraga F., Sepúlveda R.V., Carrasquel-Ursulaez W., Rojas M., Carmona E., Lorenzo-Ceballos Y., González-Nilo F., González C., Latorre R, & Torres Y.P. (2019). The molecular nature of the 17β-Estradiol binding site in the voltage- and Ca2+-activated K+ (BK) channel β1 subunit. Scientific Reports, 9, 9965.
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2

Membrane Protein Localization Assay

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Methyl-β-cyclodextrin (MβCD), water-soluble cholesterol (CLR), membrane-impermeant E2-BSA-FITC, and soluble 17β-Estradiol (E2) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Extracellular rabbit anti-MaxiK-α was purchased from Alomone Labs (Jerusalem, Israel) and extracellular rabbit anti-MaxiK-β1 from Novus Biologicals (Littleton, CO, United States). Secondary antibodies anti-rabbit Alexa Fluor 488, 568, and nuclear marker 4ʹ,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) were acquired from Invitrogen (Waltham, MA, United States).
Granados S.T., Latorre R, & Torres Y.P. (2021). The Membrane Cholesterol Modulates the Interaction Between 17-βEstradiol and the BK Channel. Frontiers in Pharmacology, 12, 687360.
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