Lanthanum la3 chloride

Angiotensin II (AngII) was obtained from Alomone (Jerusalem, Israel); Fasudil, Y-27632, ethidium (Etd⁺) bromide, lanthanum (La³⁺) chloride, Losartan and malondialdehyde (MDA) were obtained from Sigma-Aldrich (St. Louis, MO, USA); Gap27—a peptide that inhibits Cx43 HCs—was obtained from NeoMPS, SA (Strasbourg, France); A740003—a specific P2X₇Rblocker—was obtained from Tocris Biosciences (Bristol, UK). The monoclonal anti-α-tubulin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA); the polyclonal anti-phosphorilated-MYPT1 (Thr696) antibody was obtained from Merck Millipore (Darmstadt, Germany); the monoclonal anti-MYPT1 antibody was obtained from BD Transduction Laboratories (San José, CA, USA); the monoclonal anti-unphosphorylated Cx43 antibody was obtained from Invitrogen (Carlsbad, CA, USA); a polyclonal anti-Panx1 antibody described previously [65 (link)] was used; the polyclonal anti-P2X₇R antibody was obtained from Abcam (Cambridge, UK); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

Angiotensin II (Ang II) was obtained from Alomone (Jerusalem, Israel), lanthanum (La³⁺) chloride, penicillin (10,000 U) and streptomycin (10 mg/mL), carbenoxolone (CBX), the monoclonal anti-α-tubulin antibody, probenecid (PBC) and malondialdehyde (MDA) were obtained from Sigma‒Aldrich (St. Louis, MO, United States); ¹⁰panx1 (WRQAAFVDSY, first extracellular loop domain of Panx1) was obtained from GenScript (New Jersey, United States); and A740003, a specific P2X7 receptor blocker, was obtained from Tocris Biosciences (Bristol, United Kingdom). The monoclonal anti-unphosphorylated Panx1 antibody was obtained from Invitrogen (Carlsbad, CA, United States); and anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States). HEPES, ATP, NaCl, KCl, CaCl₂, MgCl₂ and glucose were obtained from MERCK (Darmstadt, Germany). Fetal bovine serum (FBS) was purchased from HyClone (Logan, UT, United States), whereas trypsin 10X, Hank’s solution, ATP determination kit, FURA-2 AM Dulbecco’s modified Eagle’s medium (DMEM), phosphate-buffered saline (PBS) and ethidium (Etd) bromide (10 mg/mL) were purchased from Thermo Fisher Scientific (Waltham, MA, United States). 4′,6-Diamidino-2-phenylindole (DAPI) was obtained from Abcam (Cambridge, United Kingdom).