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Anti actinin h 300 sc 15335

Manufactured by Santa Cruz Biotechnology
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The Anti-Actinin H-300 sc-15335 is a primary antibody produced in rabbits that recognizes the H-300 epitope of the actinin protein. It is suitable for use in various immunoassay applications.

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Lab products found in correlation

Anti gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions) Anti mettl14 antibody hpa038002, by Merck Group (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Bis tris acrylamide gel, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

H-300 (126 citations) Sc-15335 (4 citations)

2 protocols using anti actinin h 300 sc 15335

1

Western Blot Analysis of m6A Regulators

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30 μg of whole cell extract was separated by 10% SDS-PAGE and electroblotted to nitrocellulose membrane (Protran, S&S). Immunoblots were incubated with antibodies Anti-FLAG M2 F3165 (Sigma-Aldrich), Anti-WTAP 60188-1-Ig (Proteintech), Anti-METTL3 [EPR18810] (Abcam), Anti-METTL14 antibody HPA038002 (Sigma Aldrich), anti-Actinin H-300 sc-15335 (Santa Cruz Biotechnology), anti-GAPDH sc-25778 (Santa Cruz Biotechnology), anti-YTHDF2 NBP2-31785 (Novus Biological) and anti-eIF3A ab86146 (Abcam).
Sorci M., Ianniello Z., Cruciani S., Larivera S., Ginistrelli L.C., Capuano E., Marchioni M., Fazi F, & Fatica A. (2018). METTL3 regulates WTAP protein homeostasis. Cell Death & Disease, 9(8), 796.
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2

Protein Extraction and Western Blot Analysis

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Cells were harvested with 50-100 μL of Protein Extraction Buffer (100 mM Tris pH 7.5, EDTA 1 mM, SDS 2%, PIC 1X (Complete, EDTA free, Roche) and incubated 20 min on ice, centrifuged at 15000 x g for 15 min at 4°C. Proteins (15-30 μg) were loaded on 4%–12% bis-tris-acrylamide gel (Life technologies) and transferred to a nitrocellulose membrane. The membrane was blocked in 5% milk and hybridized with the following antibodies, depending on the experiment.
FLAG protein sequence was detected by WB through F1804-1MG monoclonal anti-Flag M2 (Sigma-Aldrich).
Actinin and GAPDH were detected by WB through polyclonal anti-Actinin (H-300) sc-15335 (Santa Cruz Biotechnology) and monoclonal anti-GAPDH (6C5) sc-32233 (Santa Cruz Biotechnology), respectively.
Legnini I., Di Timoteo G., Rossi F., Morlando M., Briganti F., Sthandier O., Fatica A., Santini T., Andronache A., Wade M., Laneve P., Rajewsky N, & Bozzoni I. (2017). Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis. Molecular Cell, 66(1), 22-37.e9.
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