The determination of the DCNa content in filaments and 3D-printed tablets involved the dissolution of samples weighing around 37.5 mg in volumetric flasks (25 mL) filled with a mixture of methanol and water (70:30), placed in a thermostatic bath (37 ± 0.5 °C). Repeated cycles of shaking and ultrasonication (Transsonic T700, Elma, Germany) were applied to facilitate the complete dissolution of the fragments. Furthermore, samples from the dissolution medium (500 µL) were withdrawn and placed in 10 mL volumetric flasks completed to volume with phosphate buffer at pH 6.8. The obtained solutions were filtered using 0.2 µm filters (Phenomenex, Torrance, CA, USA). The API contents were evaluated by means of UV-VIS spectrophotometry at 276 nm (Specord®200 Plus, Analytik Jena, Jena, Germany), using a calibration curve equation that resulted from the analysis of DCNa in phosphate buffer at pH 6.8.
Transsonic t700
Manufactured by Elma
Sourced in Germany
The Transsonic T700 is a laboratory equipment that generates high-frequency vibrations. It is designed for various applications that require controlled and consistent ultrasonic agitation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using transsonic t700
1
Quantifying DCNa in Filaments and Tablets
2
Solubility Studies of KET Filaments
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Solubility studies of KET as a reference and KET-loaded filaments were carried out by adding a surplus quantity of pure drug and drug loaded filaments in 4 mL of 0.1 M hydrochloric acid medium at pH 1.2, phosphate buffer at pH 6.8 and phosphate buffer at pH 7.4. Two fragments from each type of filament and two reference KET samples were evaluated. The samples were kept in a thermostatic bath (Raypa Trade, Barcelona, Spain) at 37 ± 0.5 °C for 48 h and intermittently agitated and ultrasonicated (Transsonic T700, Elma, Germany). The samples were subsequently centrifuged at 10,000 rpm for 10 min. The supernatant was filtered via 0.22 µm filters (Phenomenex, Torrance, CA, USA), properly diluted and analyzed for drug content by spectrophotometric method (Specord®200 Plus, AnalytikJena, Jena, Germany) at λ = 255 nm.
3
PEEK Surface Pretreatment and Characterization
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The materials used in this study are listed in Table 1. One hundred and twenty six PEEK specimens (5×5×2 mm 3 ) were sectioned from a PEEK disk with a low-speed diamond saw cutting machine (Isomet ® 1000 precision saw, Buehler, Lake Bluff, IL, USA). Subsequently, the PEEK samples were embedded in an auto-polymerizing acrylic resin (Orthojet, Lang Dental Manufacturing, Wheeling, IL, USA) and polished under copious water for 60 s with P400, P800, P1200 and P2000 grit silicon carbide paper (TOA, Samutprakarn, Thailand) in an automatic polishing device (Polishing Machine, MoPao 160E, MEGA Advance, Shandong, China) with vertical force of 25 N to produce a standard surface. All specimens were cleaned in an ultrasonic machine (Transsonic T700, Elma, Singen, Germany) for 10 min and stored in a dry place before surface pretreatment. The specimens were divided into six surface pretreatment groups: control, 70, 80, 85, 90, and 98% sulfuric acid (RCI Labscan, Bangkok, Thailand). Sulfuric acid (100 µL) was applied on 5×5 mm 2 area of each specimen for 60 s and the specimens were rinsed thoroughly with deionized water for 10 s, then ultrasonically cleaned in distilled water for 10 min and dried at room temperature.
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