The MMP activity in the conditioned medium of control and treated fibroblast was determined using the SensoLyte Plus 520 generic MMP Assay Kit (Anaspec) according to the manufacturer's instructions following 48 hours of cell treatment with 100 μg/ml of either KynA, Kyn, L-Kyn or D-Kyn. The kit is designed to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14.
Sensolyte plus 520
Manufactured by AnaSpec
Sourced in United States
The SensoLyte Plus 520 is a fluorescence-based assay kit designed for the detection and quantification of enzymatic activity. The kit utilizes a fluorogenic substrate that, upon cleavage by the target enzyme, generates a fluorescent signal which can be measured using a fluorescence microplate reader.
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Lab products found in correlation
2 protocols using sensolyte plus 520
1
MMP Activity Assay in Fibroblasts
2
Quantifying MMP-13 Activity Using Fluorometric Assay
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MMP-13 activity was determined by using SensoLyte Plus 520 specific MMP-13 assay (AnaSpec, San Jose, USA) according to the manufacturer's protocol. Detection was then carried out using a microplate reader with a filter set for excitation/emission wavelengths of 490 nm/520 nm (Thermo, New York, USA).
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