Bovine top-round muscles, porcine hindshank, and chicken whole leg muscles were collected from female calves (17 weeks old), male porcine (3 days after birth), and embryonic chickens (16 days after fertilization), minced, digested with 1% pronase (Roche, Mannheim, Germany) for 1 h at 37 °C, and centrifuged at 1000×g for 3 min. Digested tissue was passed through a 100 mm cell vacuum strainer (Millipore, Darmstadt, Germany), and the filtrate was centrifuged at 1000×g for 5 min. Pellets were suspended in Ham's F-10 + 20% FBS +1% P&S + 5 ng/mL FGF2, seeded on collagen-coated plates (Corning, Brooklyn, NY, USA), and incubated in a 5% CO2 humidified incubator at 37 °C. The bovine, porcine, and chicken MSCs isolation method was adopted from our previous report (Lim et al., 2022 ).
100 mm cell vacuum strainer
Manufactured by Merck Group
Sourced in Germany
The 100 mm cell vacuum strainer is a lab equipment product that functions as a vacuum filtration device. It is designed to separate solid particles from liquid samples using a vacuum. The core purpose of this product is to facilitate the filtration process in laboratory settings.
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Lab products found in correlation
2 protocols using 100 mm cell vacuum strainer
1
Isolation of Bovine, Porcine, and Chicken MSCs
2
Isolation and Culture of Mouse Muscle Stem Cells
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Mouse gastrocnemius (gas) muscles were collected from C57BL male mice (six weeks), minced, digested with 1% pronase (Roche, Mannheim, Germany) for 1 h at 37 °C, and centrifuged at 1000× g for 3 min. The digested tissue phase was passed through a 100 mm cell vacuum strainer (Millipore, Darmstadt, Germany), and the filtrate was centrifuged at 1000× g for 5 min. Pellets were suspended in Ham’s F-10 + 20% FBS + 1% P/S + 5 ng/mL FGF2, seeded on collagen-coated plates (Corning, Brooklyn, NY, USA), and incubated in a humidified 5% CO2 incubator at 37 °C. MSC purity was confirmed by PAX7 and MYOD immunocytochemistry.
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