Ultrascan 1000

Manufactured by Thermo Fisher Scientific

Sourced in United States

The UltraScan 1000 is a high-performance spectrophotometer designed for a variety of laboratory applications. It provides accurate and reliable absorbance measurements across a wide range of wavelengths, enabling precise sample analysis and quantification.

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Lab products found in correlation

Tecnai spirit, by Thermo Fisher Scientific (2 mentions) Xplore3d, by Thermo Fisher Scientific (1 mentions) Tecnai spirit biotwin tem, by Thermo Fisher Scientific (1 mentions)

3 protocols using ultrascan 1000

Transmission Electron Microscopy of EVs

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Fixed in 2% glutaraldehyde at room temperature for 1 h, the EVs were subsequently stained with a uranyl acetate solution (pH 4.5). Visualization and examination of the EVs were performed using an FEI Tecnai Spirit transmission electron microscope (FEI, Eindhoven, The Netherlands) operating at 120 kV. Electron micrographs were captured employing a Gatan UltraScan 1000 charge-coupled device (CCD) camera (Pleasanton, CA, USA).

Ma C., Ding R., Hao K., Du W., Xu L., Gao Q, & Yu C. (2023). Storage Stability of Blood Samples for miRNAs in Glycosylated Extracellular Vesicles. Molecules, 29(1), 103.

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Transmission Electron Microscopy Tomography

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Samples were prepared as described in TEM section. Sections (200 nm) were collected on glow discharged carbon grids, and 10 colloidal gold fiducial markers were applied. Using a linear tilt scheme, single axis tilt series were collected on a Tecnai BioTwin Spirit TEM (FEI) operated at 120 kV. Images captured over a tilt range of +/− 68° (1° increments) at 1µm defocus were recorded on a 2048 × 2048 pixel UltraScan 1000 Gatan CCD camera using the automated tomography acquisition software (Xplore 3D, FEI). The tilt series were aligned using the IMOD software package (version 4.8.40). All measurements were done using the Amira Visualization Package (version 6.0.0, FEI).

Justis A.V., Hansen B., Beare P.A., King K.B., Heinzen R.A, & Gilk S.D. (2016). Interactions between the Coxiella burnetii parasitophorous vacuole and the endoplasmic reticulum involve the host protein ORP1L. Cellular microbiology, 19(1), 10.1111/cmi.12637.

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Ultracentrifugation-Isolated EV Characterization

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EVs isolated by ultracentrifugation GlyExo-Capture were fixed with 2% glutaraldehyde for 1 hour at RT and then stained with 0.5% uranyl acetate (pH 4.5).
TEM was performed on an FEI Tecnai Spirit (FEI, Eindhoven, NL) at 120 kV. Electron micrographs were captured with a Gatan UltraScan 1000 charge-coupled device (CCD) camera (Pleasanton, USA).

Li B., Hao K., Ma C., Li Z., Li H., Du W., Sun L., Jia T., Liu A., Li Y., Xu L., Gao Q., Yang R., & Lin C. (2021). Isolation and characterization of fucosylated extracellular vesicles based on a novel high-throughput GlyExo-Capture technique.

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