Alk d5f3

Manufactured by Roche

Sourced in United States

The ALK-D5F3 is a piece of laboratory equipment used for the detection and analysis of specific proteins or molecules. It utilizes advanced technology to perform sensitive and accurate measurements. The core function of the ALK-D5F3 is to provide reliable data to support scientific research and investigations.

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Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Anti ki67, by Cell Marque (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Phospho stat3 tyr705, by Cell Signaling Technology (1 mentions) Anti rip3, by Santa Cruz Biotechnology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Crizotinib, by Pfizer (1 mentions) Rapamycin, by Pfizer (1 mentions) Autostainer link 48, by Agilent Technologies (1 mentions) Benchmark xt, by Roche (1 mentions)

Spelling variants of this product

VENTANA ALK (D5F3) CDx Assay (8 citations) ALK (D5F3) antibody (4 citations) Ventana anti-ALK (D5F3) rabbit monoclonal primary antibody (3 citations) ALK [D5F3] CDx Assay (3 citations) Anti-ALK (D5F3) rabbit monoclonal primary antibody (3 citations) VENTANA ALK (Clone D5F3) CDx kit (2 citations) ALK (Clone D5F3) CDx Kit (2 citations) ALK (D5F3) rabbit monoclonal primary antibody (2 citations) ALK (D5F3) IHC (2 citations)

2 protocols using alk d5f3

Antibody Panel for Signaling Proteins

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Antibodies to detect MET (D1C2), mTOR (7C10), LC3B, PARP, Caspase 3, Cyclin D1, phospho-ALK (Tyr1604), phospho-MET (Tyr1234/1235), AKT, phospho-AKT (Ser473), phospho-MAPK (Tyr202/204), 4EBP1, phospho-4EBP1 (Thr37/46), S6K, phospho-S6K (Ser371), RPS6, phospho-RPS6 (Ser235/236), STAT3, phospho-STAT3 (Tyr705) were from Cell Signaling (Danvers, MA, USA). Anti-RIP3 was from Santa Cruz Biotechnology (Dallas, TX, USA), Anti-Ki-67 from Cell Marque (Rocklin, CA, USA), and Anti-β-Actin from Sigma-Aldrich (München, Germany). ALK-1A4 (immunoblotting) was from Origene (Rockville, MD, USA), ALK-D5F3 (immunohistochemistry) was from Ventana Medical Systems (Tucson, AZ, USA). Crizotinib and rapamycin were purchased from Pfizer (Berlin, Germany) and ChemieTek (Indianapolis, IN, USA), respectively.

Mönch D., Bode-Erdmann S., Kalla J., Sträter J., Schwänen C., Falkenstern-Ge R., Klumpp S., Friedel G., Ott G, & Kalla C. (2018). A subgroup of pleural mesothelioma expresses ALK protein and may be targetable by combined rapamycin and crizotinib therapy. Oncotarget, 9(29), 20781-20794.

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Immunohistochemical Staining Protocol

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IHC was performed using commercially available detection kits and automated staining techniques (Benchmark XT, Ventana, Tucson, AZ; and AutostainerLink 48, Dako, Carpinteria, CA). Primary antibodies tested: Her2/neu (4B5, Ventana), pan-TRK (C17F1, Cell Signaling) and ALK (D5F3, Ventana). Cutoffs for positive staining: (3 (link)) pan-TRK, ≥1+ and ≥1% of cells, (4 (link)) Her2, ≥3+ and ≥ 10% and (5 (link)) ALK, ≥3+ and ≥ 10%.

Jonna S., Feldman R.A., Swensen J., Gatalica Z., Korn W.M., Borghaei H., Ma P.C., Nieva J., Spira A., Vanderwalde A., Wozniak A., Kim E.S, & Liu S.V. (2019). Detection of NRG1 gene fusions in solid tumors. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(16), 4966-4972.

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