Dmem ham s f 12 50 50 mix dmem f12

Mouse prostate tissues were collected, minced into small pieces, and digested with 10 mg/mL collagenase (StemCell Technologies) in DMEM/Ham’s F-12 50/50 Mix (DMEM/F12, Corning) supplemented with 10% FBS, 10 nM dihydrotestosterone (DHT, Sigma) and 10 μM Y-27632 (StemCell Technologies) at 37 °C for 90 min. Tissues were then digested with TrypLE (Gibco) supplemented with 1 nM DHT, 10 μM Y-27632 dihydrochloride at 37 °C for 15 min. Cell suspensions were passed through a 37-μm cell strainer (StemCell Technologies). Cell viability and concentration were detected using a TC Automated Cell Counter (Bio-Rad Laboratories) after Trypan blue (Gibco) staining, and cells at least 80% viability were processed. Similar experimental procedures were also described in the previous studies^{21 (link)–24} .

Fmoc‐amino acids and coupling reagents were purchased from either Anaspec (Fremont, CA) or Novabiochem (Germany). PEG500 (NH₂‐(PEG)‐COOH, 0.5 kDa), PEG2000 (2 kDa), and PEG5000 (5 kDa) were purchased from Nektar Therapeutics (Huntsville, AL). G7 PAMAM dendrimers were purchased from Dendritech Inc. (Midland, MI). CellTracker Green and PBS solution were obtained from Thermo Fisher Scientific (Waltham, MA). Fetal bovine serum (FBS) and antibiotic mixture (penicillin/streptomycin) were acquired from Invitrogen. Dulbecco's modification of Eagle's medium (DMEM), DMEM/Ham's F‐12 50/50 mix (DMEM/F‐12), and Roswell Park Memorial Institute (RPMI) medium were purchased from Corning (Manassas, VA). F‐12 nutrient mixture (Ham's F12) and trypsin‐EDTA were obtained from Gibco (Grand Island, NY). Recombinant human EpCAM was purchased from R&D Systems. All other chemicals were obtained from Sigma‐Aldrich (St. Louis, MO), unless otherwise stated.