The molecular weight of TFPs was determined by a gel permeation chromatography instrument (PL-GPC50, Varian, Inc., Shropsire, UK) based on the method of Wang et al.[16] (link). Chromatographic conditions were set as follows: a chromatographic column (7.5 × 300 mm, PLgelOlexis, Varian, Inc., Shropsire, UK), a protection column (7.5 × 50 mm, PLgelOlexis), a mobile phase of ultrapure water, a flow rate of 1.0 mL/min, and a column temperature of 40 °C. The weight average molecular weight (Mw), number average molecular weight (Mn), and polydispersion index (PDI = Mw/Mn) of each TPF sample were calculated by gel permeation chromatography software.
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