After the total protein was extracted, the protein concentration was measured with the BCA protein assay kit (Beyotime Biotechnology, China). Protein samples were electrophoresed, transferred to PVDF membranes, and blocked with 5% nonfat milk at RT for 1 h. Then the PVDF membrane was incubated with the primary antibody solution anti-RBM15 antibody (ab244374; Abcam, Shanghai, China, 1:1000), anti-TMBIM6 antibody (ab18852; Abcam, Shanghai, China, 1:1000) and anti-IGF2BP3 antibody (ab177477; Abcam, Shanghai, China, 1:1000) overnight at 4 °C. Monoclonal anti-rabbit IgG (RS0002; Immunoway, Plano, USA, 1:5000) or monoclonal anti-mouse IgG (3420; Abcam, Shanghai, China, 1:5000) were incubated with the PVDF membrane for a secondary step 1 h at room temperature. Finally, chemiluminescence (ECL) detection reagents (Beyotime Biotechnology, China) were prepared under dark conditions to detect blots.
Monoclonal anti mouse igg 3420
Manufactured by Abcam
Sourced in United States
Monoclonal anti-mouse IgG (3420) is a laboratory reagent used to detect and quantify mouse immunoglobulin G (IgG) in various experimental and analytical techniques.
Automatically generated - may contain errors
3 protocols using monoclonal anti mouse igg 3420
1
Western Blot Analysis of Protein Targets
2
Protein Quantification and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the total protein was extracted, the protein concentration was measured with the BCA protein assay kit (Beyotime Biotechnology, China). Then the PVDF membrane was incubated with the primary antibody solution anti-RBM15 antibody (ab244374; Abcam, Shanghai, China, 1:1,000), anti-TMBIM6 antibody (ab18852; Abcam, Shanghai, China, 1:1,000) and anti-IGF2BP3 antibody (ab177477; Abcam, Shanghai, China, 1:1,000) overnight at 4 °C. Monoclonal anti-rabbit IgG (RS0002; Immunoway, Plano, USA, 1:5,000) or monoclonal anti-mouse IgG (3420; Abcam, Shanghai, China, 1:5,000) were incubated with the PVDF membrane for a secondary step 1 h at room temperature. Finally, chemiluminescence (ECL) detection reagents (Beyotime Biotechnology, China) were prepared under dark conditions to detect blots.
3
Western Blot Analysis of Protein Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the total protein was extracted, the protein concentration was measured with the BCA protein assay kit (Beyotime Biotechnology, China). Protein samples were electrophoresed, transferred to PVDF membranes, and blocked with 5% nonfat milk at RT for 1 h. Then the PVDF membrane was incubated with the primary antibody solution anti-RBM15 antibody (ab244374; Abcam, Shanghai, China, 1:1,000), anti-TMBIM6 antibody (ab18852; Abcam, Shanghai, China, 1:1,000) and anti-IGF2BP3 antibody (ab177477; Abcam, Shanghai, China, 1:1,000) overnight at 4°C. Monoclonal anti-rabbit IgG (RS0002; Immunoway, Plano, USA, 1:5,000) or monoclonal anti-mouse IgG (3420; Abcam, Shanghai, China, 1:5,000) were incubated with the PVDF membrane for a secondary step 1 h at room temperature. Finally, chemiluminescence (ECL) detection reagents (Beyotime Biotechnology, China) were prepared under dark conditions to detect blots.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!