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Anti caspase 1 d7f10

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-Caspase-1 (D7F10) is a primary antibody that recognizes the p20 subunit of human Caspase-1. Caspase-1 is a cysteine protease that plays a central role in the inflammatory response by cleaving and activating pro-inflammatory cytokines such as IL-1β and IL-18.

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2 protocols using anti caspase 1 d7f10

1

Apoptosis and Pyroptosis Pathway Analysis

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The following antibodies were used: anti-Caspase-1 (D7F10 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-4 (4450 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-5 (D3G4W from Cell Signaling Technology, Danvers, MA, USA); anti-actin (C4 from MP Biomedicals); anti-GSDMD (G7422 from Sigma-Aldrich, St. Louis, MO, USA), anti-GSDMD N-term (E7H9G from Cell Signaling Technology, Danvers, MA, USA), anti-IL-1β (MAB601, R&D Systems, Inc., Minneapolis, MN, USA). All cell culture media reagents were purchased from Thermo Fisher (Waltham, MA, USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Inflammasome Signaling Pathway Analysis

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The following antibodies were used: anti-Caspase-1 (D7F10 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-4 (4450 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-5 (D3G4W from Cell Signaling Technology, Danvers, MA, USA); anti-actin (C4 from MP Biomedicals); anti-GSDMD (G7422 from Sigma-Aldrich, St. Louis, MO, USA), anti-GSDMD N-term (E7H9G from Cell Signaling Technology, Danvers, MA, USA), anti-IL-1β (MAB601, R&D Systems, Inc., Minneapolis, MN, USA), anti-IL-1β cleaved, Asp116 (PA5-105048 from Thermo Fisher, Waltham, MA, USA). All cell culture media reagents were purchased from Thermo Fisher (Waltham, MA, USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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