Diclofenac sodium salt (DIC), naproxen sodium salt (NAP), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), Trizma® base, sodium chloride, sodium hydroxide, potassium phosphate monobasic, ascorbic acid, 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt hydrate (PTS), methyl viologen dichloride hydrate (MV), Sephadex G-25® medium, and nonaethylene glycol monododecyl ether (polidocanol) were supplied by Sigma-Aldrich Co. (St. Louis, MO, USA). Cholesterol from ovine wool (CHOL), 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (sodium salt) (TMCL), cardiolipin sodium salt from bovine hearth (CL), and L-α-phosphatidylinositol sodium salt from bovine liver (PI) was supplied by Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Chloroform and methanol were obtained from VWR International S.A.S. (Fontenais-sous-Bois, France). Ammonium heptamolybdate tetrahydrate and hydrochloric acid were acquired from E. Merck (Darmstadt, Germany) and Fisher Scientific UK (Loughborough, UK), respectively. Perchloric acid was purchased by Riedel-de Haen (Seelze, Germany).
All assays were performed mimicking physiological conditions using Tris-HCl buffer (pH 7.4). It was prepared by dissolving Trizma® base (10 mM) and NaCl (150 mM) in double deionized water (κ < 0.1 μS.cm−1) and adjusting the pH value using an HCl solution (1 M).
All assays were performed mimicking physiological conditions using Tris-HCl buffer (pH 7.4). It was prepared by dissolving Trizma® base (10 mM) and NaCl (150 mM) in double deionized water (κ < 0.1 μS.cm−1) and adjusting the pH value using an HCl solution (1 M).