The apoptosis rate of cells was detected using AnnexinV/propidium iodide detection kit (Keygene, Nanjing, China). Briefly, after transfection, cells were harvested and then stained with 5 µL Annexin V-FITC and 5 µL PI for 15 minutes at 4°C. Then, the stained cells were analyzed by flow cytometer (BD Biosciences, San Jose, CA, USA).
Annexinv propidium iodide detection kit
Manufactured by Keygene
Sourced in China
The AnnexinV/propidium iodide detection kit is a laboratory assay used to detect and quantify apoptosis, or programmed cell death, in cell samples. The kit utilizes the binding properties of the protein AnnexinV and the DNA-binding dye propidium iodide to distinguish between viable, apoptotic, and necrotic cells. This information can be used to study various cellular processes and responses.
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Lab products found in correlation
6 protocols using annexinv propidium iodide detection kit
1
Apoptosis Detection by Flow Cytometry
2
Apoptosis and Cell-Cycle Analysis
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For apoptosis and cell-cycle assay, cells were transfected with siTUG1, then treated with drugs for 24 h or not before collected. Cell apoptosis assay was conducted by using AnnexinV/propidium iodide detection kit (Keygene, Nanjing, China). For cell-cycle assay, cells were collected and fixed in 70% ethanol at 4 °C for 16 h and then stained with propidium iodide.
3
Apoptosis and Cell Cycle Analysis
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Cells were treated with drugs for 24h after transfection, and then collected for apoptosis and cell-cycle assay. Cell apoptosis assay was performed by using AnnexinV/propidium iodide detection kit (Keygene, Nanjing, China). For cell-cycle assay, the cells were collected and fixed in 70% ethanol at 4°C for 16h and then stained with propidium iodide.
4
Apoptosis Assay with siRNA and Overexpression
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For apoptosis assay, cells were transfected with si-HOTTIP and pcDNA3.1-HOTTIP plasmid, respectively, then all cells groups were treated with chemo-drugs for 24 h before being collected. Annexin V/propidium iodide detection kit (Keygene, Nanjing, China) was used for cell apoptosis assay. As there were spontaneous green fluorescence with cells after transfection, so the gate in detecting by flow cytometry should be regulated in cell apoptosis with negative staining and blank control.
5
Apoptosis and Cell Cycle Assay
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Cells were treated with drugs for 24 h after transfection and then collected for apoptosis and cell cycle assay. Cell apoptosis assay was performed using Annexin V/propidium iodide detection kit (Keygene, Nanjing, China). For cell cycle assay, the cells were collected and fixed in 70% ethanol at 4°C for 16 h and then stained with propidium iodide.
6
Cell Cycle and Apoptosis Analysis in PTC Cells
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Cell cycle and apoptosis analyses of PTC cells were carried out using flow cytometry. K1 and B-CPAP cell lines after transfection were fixed with 75% ethanol for at least 1 h and washed using D-Hanks. Then the cells were stained with PI (Sigma–Aldrich, St. Louis, Missouri, U.S.A.) for 30 min. After the incubation at 4°C, the cells were detected using FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, U.S.A.). The obtained data were subsequently analyzed employing CellQuest software (BD Biosciences, Franklin Lakes, NJ, U.S.A.).
For the analysis on cell apoptosis, the apoptosis of the transfected cells were evaluated using Annexin V/propidium iodide detection kit (KeyGene, Nanjing, China) following the manufacturer’s instructions. FACS Calibur flow cytometer was used to detect the cells, and the data were analyzed using CellQuest software. Three separately prepared cell cultures were subjected to analysis in triplicate.
For the analysis on cell apoptosis, the apoptosis of the transfected cells were evaluated using Annexin V/propidium iodide detection kit (KeyGene, Nanjing, China) following the manufacturer’s instructions. FACS Calibur flow cytometer was used to detect the cells, and the data were analyzed using CellQuest software. Three separately prepared cell cultures were subjected to analysis in triplicate.
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