Soybean phosphatidylcholine (SPC, purity > 90%) was a gift from Lipoid (Ludwigshafen, Germany). Imiquimod (purity >98%) was a gift from Laboratorio Lazar (Buenos Aires, Argentina). MONTANIDE™ ISA 763 A VG was from Seppic (Puteaux, France). Ficoll was from GE Healthcare (Munich, Germany). Hypaque was from Winthrop Products (Buenos Aires, Argentina). Roswell Park Memorial Institute (RPMI) 1640 medium was from Gibco, Life Technologies (New York, USA). Antibiotic/antimycotic solution (penicillin 10,000 IU/mL, streptomycin sulfate 10 mg/mL, amphotericin B 25 μg/mL), glutamine, and trypsin/ethylenediamine tetraacetic acid were from PAA Laboratories GmbH (Pasching, Austria). Fetal bovine serum (FBS) was from Internegocios, Cordoba, Argentina. Concanavalin A (ConA), Phorbol 12-myristate 13-acetate (PMA), Laurdan, saponin, Carboxy fluorescein succimidil ester (CFSE), 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) were from Sigma-Aldrich (San Diego, USA). The other reagents were analytic grade from Anedra, Research AG (Buenos Aires, Argentina).
Trypsin ethylenediamine tetraacetic acid
Manufactured by GE Healthcare
Sourced in Austria
Trypsin/ethylenediamine tetraacetic acid is a laboratory reagent used for the dissociation and detachment of adherent cells from cell culture surfaces. It functions by enzymatically cleaving cell-cell and cell-substrate adhesion proteins.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions)
Amphotericin b, by GE Healthcare (2 mentions)
Glutamine, by GE Healthcare (2 mentions)
Soybean phosphatidylcholine, by Lipoid (1 mentions)
Montanide isa763 a vg, by Seppic (1 mentions)
2 2 azino bis 3 ethylbenzthiazoline 6 sulphonic acid abts, by Merck Group (1 mentions)
Laurdan, by Merck Group (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ficoll, by GE Healthcare (1 mentions)
Saponin, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Antibiotic antimycotic solution, by GE Healthcare (1 mentions)
Streptomycin sulfate, by GE Healthcare (1 mentions)
Dulbecco s modified eagle s medium, by PAN Biotech (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Line 3t3 swiss albino ccl 92, by American Type Culture Collection (1 mentions)
Molybdenum oxide, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
3 protocols using trypsin ethylenediamine tetraacetic acid
1
Soybean Phosphatidylcholine-Based Immunomodulatory Formulations
2
Culturing 3T3 Fibroblasts for Metabolic Assays
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The mouse 3T3 fibroblast cells (line 3T3-Swiss albino CCL-92™; American Type Culture Collection, Manassas, VA, USA) were routinely maintained in a humidified incubator with an atmosphere of 5% CO2 in air at 37°C with fresh medium changed every 2 days. The cells were cultured in Dulbecco’s Modified Eagle’s Medium (Pan-Biotech, Aidenbach, Germany) supplemented with 10% fetal bovine serum (PAA Laboratories, Pasching, Austria) and penicillin–streptomycin (100 IU/mL and 100 μg/mL, respectively; Sigma-Aldrich). When the cells reached 80%–90% confluence, they were suspended using trypsin–ethylenediaminetetraacetic acid (PAA Laboratories). For detection of metabolic activity, the scaffolds were seeded with 3T3 fibroblasts at a density of 3×103 cells/cm2.
3
Liposomal Delivery of Vancomycin
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T was obtained from Euma, Buenos Aires, Argentina (density of T was considered 0,915 g/ml). Soybean phosphatidylcholine (SPC, purity >90%) was a gift from Lipoid, Ludwigshafen, Germany. Polysorbate 80 (P80), molybdenum oxide, ascorbic acid, Sephadex G-25, 6-dodecanoyl-N, N-dimethyl-2-naphthylamine (laurdan), vancomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and crystal violet were purchased from Sigma-Aldrich (St Louis, MO, USA). Roswell Park Memorial Institute (RPMI) 1640 medium was from Gibco (Buenos Aires, Argentina).
Fetal bovine serum (FBS), antibiotic/antimycotic solution (penicillin 10,000 IU/ml, streptomycin sulphate 10 mg/mL, amphotericin B 25 µg/ml), glutamine, and trypsin/ethylenediaminetetraacetic acid were from PAA Laboratories GmbH (Pasching, Austria). Tryptic soy broth (TSB) and bacteriological agar were acquired from Britania, Buenos Aires, Argentina. The other reagents were of analytic grade from Anedra (Buenos Aires, Argentina), Research AG (Tigre, Buenos Aires, Argentina).
Fetal bovine serum (FBS), antibiotic/antimycotic solution (penicillin 10,000 IU/ml, streptomycin sulphate 10 mg/mL, amphotericin B 25 µg/ml), glutamine, and trypsin/ethylenediaminetetraacetic acid were from PAA Laboratories GmbH (Pasching, Austria). Tryptic soy broth (TSB) and bacteriological agar were acquired from Britania, Buenos Aires, Argentina. The other reagents were of analytic grade from Anedra (Buenos Aires, Argentina), Research AG (Tigre, Buenos Aires, Argentina).
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