All cells were cultured in a humidified incubator at 37 °C with 5% CO2. Vector control and GSDMD KD THP-1 cells were generated by lentiviral shRNA-mediated targeting and were generously provided by Dr. Amal Amer at The Ohio State University. Cells were maintained in RPMI 1640 medium (Fisher Scientific) supplemented with 10% EquaFETAL bovine serum (Atlas Biologicals). THP-1 were differentiated into macrophages by treatment with 25 nM phorbol myristate acetate (PMA) for 3 days as previously described68 (link) to allow for differentiation into macrophages. Infection of THP-1 cells with PR8 or H3N2 viruses was done at an MOI of 10 for 48 h prior to collection of cells for Western blotting or cellular media for measurement of cytokines and LDH release. MDCK cells (BEI Resources, NR-2628) were grown in DMEM (Fisher Scientific) supplemented with 10% EquaFETAL bovine serum.
Mdck cells
Manufactured by BEI Resources
MDCK cells are a commonly used cell line derived from the kidney of a normal adult female cocker spaniel dog. They are an epithelial cell line that retains many characteristics of normal kidney tubule cells, making them a valuable model for studying various biological processes and evaluating the effects of drugs or other substances on kidney function.
Automatically generated - may contain errors
2 protocols using mdck cells
1
THP-1 Macrophage Infection with Influenza Viruses
2
Generation of IFITM-Deficient Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Control and IFITM3 knockdown A549 cells were generated by lentiviral shRNA-mediated targeting using previously described constructs and methods38 (link). THP-1 IFITM3 knockout cells were generated via CRISPR-Cas9 targeting (provided by Dr. Anasuya Sarkar of the Ohio State University). HeLa IFITM1/2/3 knockout and HAP1 IFITM3 knockout were purchased from ATCC (CRL-3452) and Horizon Discovery Biosciences (HZGHC004186c010), respectively. MDCK cells were obtained from BEI Resources (NR-2628). Cells were maintained in DMEM medium (Fisher scientific) supplemented with 10% EquaFETAL bovine serum (Atlas Biologicals) except for the THP-1 cells which were maintained in RPMI 1640 medium (Fisher scientific) supplemented with 10% EquaFETAL bovine serum (Atlas Biologicals). All cells were cultured in a humidified incubator at 37 °C with 5% CO2.
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