Sodium taurocholate

Melting points were determined on open glass capillaries using Gallenkamp apparatus and are uncorrected. Infrared spectra were measured on a Shimadzu FT-IR 8400s infrared spectrophotometer (cm⁻¹) using KBr disks, Faculty of Pharmacy, Cairo University, Cairo, Egypt. ¹H and ¹³C-NMR spectra were determined in DMSO-d₆ with Bruker Avance-400 spectrometer operating at 400 MHz and 100 MHz, respectively, at Applied Nucleic Acid Research Centre (ANARC), Faculty of Science, Zagazig University, Zagazig, Egypt. TLC was performed on 2.5 × 5 cm aluminum plates coated with silicagel 60F-254 (Merk, Germany) in an appropriate solvent. Span 60 was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Sodium taurocholate (STC) and sodium deoxycholate (SDC) were purchased from BASF Co. (Florham Park, NJ, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [methoxy(polyethylene glycol)-2000] (DSPE–mPEG-2000) was a generous gift from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was obtained from ITX Biomedicals (Santa Ana, CA, USA). Sodium hydroxide, potassium dihydrogen orthophosphate and absolute ethanol were purchased from El-Nasr Chemical Co., Cairo, Egypt. The Spectra/PoreVR dialysis membrane (12,000–14,000 molecular weight cut off) was purchased from Spectrum Laboratories Inc., Los Angeles, CA, USA. All chemicals and solvents were of HPLC grade and were used as received.