Acetonitrile

Methacrylic acid (MAA), divinylbenzene (DVB), 2,2-azobis (isobutyronitrile) (AIBN) were purchased from Shanghai Aladdin Chemistry Co. Ltd., acetonitrile (ACN) and methanol were purchased from Shanghai Lingfeng Chemical Reagent Co. Ltd., ethanol was purchased from Shanghai Zhenxing Chemistry Co. Ltd., iron (III) chloride hexahydrate (FeCl₃·6H2O), potassium chloride (KCl), magnesium chloride hexahydrate (MgCl₂·6H₂O), edetate disodium (EDTANa₂·2H₂O), Tris, hydrochloric acid (HCl) and sucrose were all purchased from Sinopharm Chemical Reagent Co. Ltd.. Protease inhibitor, high glucose DMEM medium, fetal bovine serum (FBS), penicillin–streptomycin, phosphate buffer saline (PBS) and trypsin enzyme were purchased from Thermo Fisher Scientific (Waltham, MA, USA). CCK-8 kit was purchased from Dojindo Laboratories (Tokyo, Japan). DAPI, Lyso-tracker green and cell viability (staining of living and dead cells) assay kit were purchased from KeyGen Biotech (Nanjing, Jiangsu, China). Triton X-100 and γ-H2AX antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cypate was synthesized as previously described [46 (link)]. Deionized water used in all experiments was obtained from Milli-Q water system.

All pesticide standards were purchased from Shanghai Anpu Experimental Technology Co. (Shanghai, China). HPLC grade acetonitrile and methanol were obtained by Merck Co. (Darmstadt, Germany). HPLC grade formic acid was purchased from Anaqua Chemicals Supply (Wilmington, NC, USA). Anhydrous magnesium sulfate (MgSO₄) was obtained from RHAWN (Shanghai, China). MWCNT (O.D. 20–30 nm, L. 0.5–2 μm, O.D. 10–20 nm, L. 0.5–2 μm, O.D. 10–20 nm, L. 10–30 μm) and g-MWCNTs (O.D. 10–20 nm, L. 5–30 μm) were obtained by XFNANO (Nanjing, China). g-MWCNTs (O.D. 30–50 nm, L. 10–20 μm), MWCNTs-COOH (O.D. 30–50 nm, L. 10–20 μm), MWCNTs-OH (O.D. 30–50 nm, L. 10–20 μm), MWCNTs-NH₂ (O.D. 8–15 nm, L. −50 μm), and sorbents ZrO₂ were purchased from Aladdin Industrial Corporation (Shanghai, China). PSA and C18 were obtained by Agilent Technologies (Tianjin, China). 2 mL Agela Cleanert MAS-Q purification tubes (PSA, 50 mg; C18, 50 mg; MgSO₄, 150 mg) were purchased from Bonna-Agela Technologies Co. (Tianjin, China). Sodium chloride (NaCl) and AR-acetonitrile were purchased from Shanghai Lingfeng Chemical Reagent Co. (Shanghai, China).
A separate standard solution for each pesticide was prepared in acetonitrile at a concentration of 1000 mg/L. Then, the mixed standard solutions were prepared in methanol. All standard solutions were stored in a refrigerator at 4 °C for use in subsequent experiments.