Bio plex pro cytokine assay kit

Manufactured by Bio-Rad

Sourced in United States

The Bio-Plex Pro™ Cytokine Assay Kit is a multiplex immunoassay designed for the simultaneous quantification of multiple cytokines, chemokines, and growth factors in biological samples. The kit utilizes magnetic bead-based technology to capture and detect the target analytes.

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Lab products found in correlation

Duoset elisa kit, by R&D Systems (1 mentions)

Close spelling variants of this product

Bio-Plex (286 citations) Bio-Plex assay (73 citations) Bio-Plex kit (41 citations) Cytokine assay kits (2 citations)

5 protocols using bio plex pro cytokine assay kit

Cytokine Quantification in Cell Culture

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TNF-α and IL-6 levels in culture supernatants were determined using a commercially available Bio-Plex Pro™ Cytokine Assay Kit (Bio-Rad, USA) according to the manufacturer’s instructions.

Lee K.H., Jeong J., Woo J., Lee C.H, & Yoo C.G. (2018). Globular Adiponectin Exerts a Pro-Inflammatory Effect via IκB/NF-κB Pathway Activation and Anti-Inflammatory Effect by IRAK-1 Downregulation. Molecules and Cells, 41(8), 762-770.

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Quantifying IL-8 in Cell Culture

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IL-8 levels in culture supernatants were determined using a commercially available Bio-Plex Pro™ Cytokine Assay Kit (Bio-Rad, USA) according to the manufacturer’s instructions.

Lee K.H., Lee C.H., Woo J., Jeong J., Jang A.H, & Yoo C.G. (2018). Cigarette Smoke Extract Enhances IL-17A-Induced IL-8 Production via Up-Regulation of IL-17R in Human Bronchial Epithelial Cells. Molecules and Cells, 41(4), 282-289.

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Cytokine Quantification in Cell Culture

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The levels of cytokine in the culture supernatants were determined using a Bio-Plex Pro™ cytokine assay kit (Bio-Rad, Hercules, CA, USA).

Lee K.H., Woo J., Kim J., Lee C.H, & Yoo C.G. (2022). YPL-001 Shows Various Beneficial Effects against Cigarette Smoke Extract-Induced Emphysema Formation: Anti-Inflammatory, Anti-Oxidative, and Anti-Apoptotic Effects. Antioxidants, 12(1), 15.

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Cytokine Profiling of Immune Cells

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Culture supernatants were collected and centrifuged for 5 min at 15,000× g. Cytokine levels (CD14 + monocytes: IL-1β, IL-4, IL-6, IL-7, IL-10, IL-12, IL-15, IFN-γ, and TNF-α; CD4 + /CD8 + T cells and iPS-MLs: IL-1β, IL-6, IL-10, IL-15, and IFN-γ) in culture supernatants were measured using the Bio-Plex Pro Cytokine Assay kit (Bio-Rad).

Suenaga G., Ikeda T., Masuda T., Motokawa H., Yamashita T., Takamatsu K., Misumi Y., Ueda M., Matsui H., Senju S, & Ando Y. (2017). Inflammatory state exists in familial amyloid polyneuropathy that may be triggered by mutated transthyretin. Scientific Reports, 7, 1579.

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Cytokine Profiling in Lung Inflammation

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The levels of interleukin-8 (IL-8), keratinocytes-derived chemokine (KC), macrophage inflammatory protein 2 (MIP2), tumor growth factor-α (TNF-α), and IL-6 in cell culture supernatants or in BALF were measured using a commercially available DuoSet ELISA kit (R&D System, Minneapolis, MN, USA). The concentrations of TNF-α, IL-6, and IL-1β in BALF were measured using a Bio-Plex Pro™ cytokine assay kit (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

Heo E.Y., Lee K.H., Woo J., Kim J., Lee C.H., Lee K.J., Kim Y.K, & Yoo C.G. (2022). Cereblon Deficiency Contributes to the Development of Elastase-Induced Emphysema by Enhancing NF-κB Activation. Antioxidants, 11(10), 1980.

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