We generated a pDisplay Vector (Life Technologies) encoding His-tagged IL-15/IL-15Rα (IL-15 linked to IL-15P sushi)28 (link). Following transfection of 293-F cells, we purified protein from culture supernatant using standard Cobalt agarose resin (Thermo Scientific) according to protocol.
Cobalt agarose resin
Manufactured by Thermo Fisher Scientific
Cobalt agarose resin is a laboratory product used for the purification of histidine-tagged proteins. It utilizes the affinity between cobalt ions and the histidine tag to capture and isolate the target proteins from complex mixtures. The resin provides a stable and reversible binding platform for efficient protein recovery.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cobalt agarose resin
1
Purification of His-tagged IL-15/IL-15Rα
2
Purification of His-tagged IL-15/IL-15Rα
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