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Rat anti p2ry12

Manufactured by BioLegend
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Rat anti-P2RY12 is a monoclonal antibody that recognizes the P2RY12 receptor, which is a G-protein coupled receptor expressed on the surface of platelets and microglia. The antibody can be used to detect and study the P2RY12 receptor in various experimental applications.

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Lab products found in correlation

Rabbit anti iba1, by Fujifilm (2 mentions) Mouse anti tnf α, by Abcam (1 mentions) Rabbit anti inos, by BD (1 mentions) Mouse anti cox 2, by BD (1 mentions) Rabbit anti mcp 1, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Rabbit anti neun, by Abcam (1 mentions) Mouse anti gapdh, by Merck Group (1 mentions) Trizol rna isolation reagent, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfap, by Merck Group (1 mentions) Fluoromount g mounting medium, by Southern Biotech (1 mentions) Goat anti olig2, by R&D Systems (1 mentions) Trueblack lipofuscin autofluorescence quencher, by Biotium (1 mentions) Rabbit anti tnf α, by Abcam (1 mentions) Mouse anti mhc 2, by Abcam (1 mentions) Isolectin gs ib4 alexa fluor 594 conjugate, by Thermo Fisher Scientific (1 mentions) Rabbit anti tmem119, by Abcam (1 mentions) Rabbit anti cd68, by Abcam (1 mentions) Rabbit anti ki67, by Abcam (1 mentions) Cd103, by Thermo Fisher Scientific (1 mentions)

4 protocols using rat anti p2ry12

1

Immunohistochemistry and Immunoblotting Protocols

Cited 1 time
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Immunohistochemistry and immunoblotting were performed as previously described 4 (link), 34 (link). The following antibodies for immunohistochemistry were used: rabbit anti-GFP (#A6455, Invitrogen), rabbit anti-MCP-1 (#500-P113, PeproTech), mouse anti-TNFα (#ab8348, Abcam), rabbit anti-NeuN (#ab177487, Abcam), rabbit anti-Iba1 (#019-19741, Wako), rabbit anti-Ki67 (#ab15580, Abcam), rat anti-CD68 (#FA-11, Abcam), rabbit anti-Tmem119 (#ab209064, Abcam), rat anti-P2RY12 (#848001, BioLegend), rabbit anti-Synaptotagmin 1 (#105003, Synaptic Systems), mouse anti-MHC-II (#ab55152, Abcam), and mouse anti-PSD-95 (#MAB1596, EMD Millipore). Species-specific secondary antibodies were conjugated with Alexa Fluor488, 594 or 647 (Molecular Probes, Invitrogen). The antibodies for immunoblotting were: rabbit anti-iNOS (#610332, BD PharMingen), mouse anti-COX2 (#610204, BD PharMingen), and mouse anti-GAPDH (#MAB374, EMD Millipore).
Chen H.R., Chen C.W., Kuo Y.M., Chen B., Kuan I.S., Huang H., Lee J., Anthony N., Kuan C.Y, & Sun Y.Y. (2022). Monocytes promote acute neuroinflammation and become pathological microglia in neonatal hypoxic-ischemic brain injury. Theranostics, 12(2), 512-529.
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2

Multiparametric Analysis of Neurodegeneration

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Human/murine phospho-tau pSer202/Thr205 (AT8, Cat# MN1020), Tau46 (Cat# 13-6400), Alexa Fluor dye-labeled cross-absorbed donkey secondary antibodies, and TRIzol RNA isolation reagent (Cat# 15596026) were purchased from ThermoFisher Scientific. Goat anti-Olig2 (Cat# AF2418) and GAD1 (Cat# AF2086) polyclonal antibody were purchased from R&D Systems. Rabbit anti-GFAP (Cat# G9269), WFS1 (Cat# 1158-1-AP), and Aβ (Cat# Ab2539) polyclonal antibodies were purchased from Sigma-Aldrich, Proteintech, Abcam, and DAKO, respectively. Rat anti-P2RY12 (Cat# 848002) was purchased from BioLegend. RNAscope HiPlex Ancillary Kit (Cat# 324120) and human-specific RNA probes were purchased from Advanced Cell Diagnostics. TrueBlack lipofuscin autofluorescence quencher (Cat# 23007) was purchased from Biotium. Fluoromount-G Mounting Medium (Cat# 0100-01) was purchased from SouthernBiotech.
Chen S., Chang Y., Li L., Acosta D., Li Y., Guo Q., Wang C., Turkes E., Morrison C., Julian D., Hester M.E., Scharre D.W., Santiskulvong C., Song S.X., Plummer J.T., Serrano G.E., Beach T.G., Duff K.E., Ma Q, & Fu H. (2022). Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease. Acta Neuropathologica Communications, 10, 188.
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3

Immunohistochemical Analysis of Microglia in Mouse Brain

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Brains from indicated littermates were fixed using 4% paraformaldehyde (PFA) at 4°C overnight with gentle agitation, cryopreserved in 30% sucrose, frozen, and finally stored at −20°C until use. For staining, 20-μm cryosections were made and incubated in blocking/permeabilization solution containing 3% normal goat serum (NGS) and 0.2% Triton-X in PBS. The sections were treated overnight with appropriate primary antibodies diluted in 1% NGS/0.2% Triton X-100/PBS followed by the corresponding secondary antibodies for 2 hours at room temperature. The following primary antibodies were used: rabbit anti-CD68 (Abcam); mouse anti-MHC-II (Abcam); rabbit anti-Ki67 (Abcam); isolectin GS-IB4, Alexa Fluor 594 conjugate (Invitrogen); rabbit anti-TNFα (Abcam); rabbit anti-Iba1 (Wako Chemicals USA Inc.); rabbit anti-Tmem119 (Abcam); and rat anti-P2RY12 (BioLegend).
Chen H.R., Sun Y.Y., Chen C.W., Kuo Y.M., Kuan I.S., Tiger Li Z.R., Short-Miller J.C., Smucker M.R, & Kuan C.Y. (2020). Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke. Science Advances, 6(35), eabb2119.
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4

Multicolor Flow Cytometry Analysis

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All used at 1:200: CD11b (Clone M1/70, Biolegend, Cat 10137), CD45 (Clone 30-F11, eBioscience, Cat 56-0451-82), MHCII (Clone M5/114.15.2, Biolegend, Cat 107626), CD86 (Clone GL1, BD Biosciences, 553691), CD80 (Clone 16-10A1, Biolegend, 104706), CD11c (Clone N418, Biolegend, Cat 117335), CD103 (Clone 2E7, eBioscience, Cat 48-1031-80), CD8a (Clone 53-6.7, Biolegend, Cat 100712), CD4 (Clone RM4-5, BD Biosciences, Cat 550954), CD69 (Clone H1.2F3, eBiosciene, Cat 12-0691-81), Ly6C (Clone HK1.4, Biolegend, Cat 128003), Ly6G (Clone 1A8, Biolegend, Cat 127616), CD160 (Clone 7H1, Biolegend, Cat 143007), Rat-anti-P2RY12 (Clone S16007D, Biolegend, Cat 848002), and Goat-anti-Rat-AlexaFluor 350 (Invitrogen, Cat A21093). WNV-specific CD8+ T cells were identified with fluorescent-labeled immunodominant Db-restricted NS4B peptide.
Funk K.E, & Klein R.S. (2019). CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis. Journal of Neuroinflammation, 16, 22.
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