A Calibur Flow Cytometer system (BD Inc., USA) was used to analyze the cell cycle distribution and apoptosis. The SH-SY5Y cells used for the detection of cell cycle distribution were stained with propidium iodide (PI). A FITC Annexin V Apoptosis Detection Kit (BD Pharmingen, USA) was used to detect apoptosis.
Calibur flow cytometer system
Manufactured by BD
Sourced in United States
The Calibur Flow Cytometer system is a laboratory instrument used for analyzing and sorting cells or particles in a fluid sample. It measures the physical and chemical characteristics of these cells or particles as they pass through a laser beam. The system collects and analyzes data on the size, granularity, and fluorescence of individual cells or particles within the sample.
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Lab products found in correlation
2 protocols using calibur flow cytometer system
1
Cell Cycle and Apoptosis Analysis
2
Cytotoxicity and Apoptosis Evaluation of BBR in PC12 Cells
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The cytotoxicity of BBR in PC12 cells was measured using an MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay, as described previously [18] (link).
Analysis of cell cycle and apoptosis by flow cytometry A Calibur Flow Cytometer system (BD Inc., U.S.) was used to analyze the cell cycle distribution and apoptosis. The PC12 cells used for detection of cell cycle distribution were stained with propidium iodide (PI). A FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen, U.S.) was used to detect apoptosis.
Analysis of cell cycle and apoptosis by flow cytometry A Calibur Flow Cytometer system (BD Inc., U.S.) was used to analyze the cell cycle distribution and apoptosis. The PC12 cells used for detection of cell cycle distribution were stained with propidium iodide (PI). A FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen, U.S.) was used to detect apoptosis.
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