Cell lines were detached from a 75-cm2 tissue culture flask by treatment with cell stripper (Cellgro; Manassas, VA.). Cells were counted, blocked and aliquots of 1.5 × 106 cells were incubated with 1.5 μg of anti-αVβ3 and anti-αVβ5 integrin antibodies (Millipore; Temecula, CA), 1.5 μg of mouse monoclonal antibody Desmoglein 2 (DSG2) (clone AH12.2, Santa Cruz Biotechnology Inc, Santa Cruz, CA) or untreated (control). Following primary antibody incubation, the cells were washed and incubated with Alexa fluor 488-conjugated goat anti-mouse immunoglobulin G (Invitrogen) diluted in PBS with 0.1% BSA. Cells were then diluted in PBS and a cytometric analysis of 10,000 events per sample was conducted using FACScan with CellQuest software (Becton Dickinson, Mountain View, CA).
Desmoglein 2 dsg2 clone ah12
Manufactured by Santa Cruz Biotechnology
Desmoglein 2 (DSG2) (clone AH12.2) is a protein used in research applications. It is a component of desmosomes, which are cell-cell adhesion structures. Desmoglein 2 plays a role in maintaining the structural integrity of tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using desmoglein 2 dsg2 clone ah12
1
Quantification of Integrin Expression
2
Flow Cytometry Analysis of Integrin and Desmoglein Expression
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Example 5
Flow Cytometry:
Cell lines were detached from a 75-cm2 tissue culture flask by treatment with cell stripper (Cellgro; Manassas, Va.). Cells were counted, blocked and aliquots of 1.5×106 cells were incubated with 1.5 μg of anti-αVβ3 and anti-αVβ5 integrin antibodies (Millipore; Temecula, Calif.), 1.5 μg of mouse monoclonal antibody Desmoglein 2 (DSG2) (clone AH12.2, Santa Cruz Biotechnology Inc, Santa Cruz, Calif.) or untreated (control). Following primary antibody incubation, the cells were washed and incubated with Alexa fluor 488-conjugated goat anti-mouse immunoglobulin G (Invitrogen) diluted in PBS with 0.1% BSA. Cells were then diluted in PBS and a cytometric analysis of 10,000 events per sample was conducted using FACScan with CellQuest software (Becton Dickinson, Mountain View, Calif.).
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