Hydrocortisone 21 hemisuccinate sodium

Metformin hydrochloride, trypsin-EDTA solution, phosphate saline buffer (PBS), dimethyl sulfoxide (DMSO), fetal calf serum (FCS), penicillin/streptomycin, insulin from bovine pancreas, Hydrocortisone 21-hemisuccinate sodium and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reagents were purchased from Sigma Aldrich, Merck KgaA (Darmstadt, Germany). The cell culture media, William’s E Medium (Gibco™; 12551032) were purchased from Gibco, Waltham, MA, USA). All reagents were analytically pure and proper for cell culture use.

Reagents used for in vitro experiments: trypsin-EDTA solution, phosphate saline buffer (PBS), dimethyl sulfoxide (DMSO), fetal calf serum (FCS), penicillin/streptomycin, insulin from bovine pancreas, hydrocortisone 21-hemisuccinate sodium, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagents were purchased from Sigma-Aldrich, Merck KgaA (Darmstadt, Germany). The cell media used, William’s E Medium (Gibco™; 12551032), was purchased from Gibco, Waltham, MA, USA, and Dulbecco’s modified Eagle’s medium (DMEM; 30–2002™) was purchased from the ATCC (American Type Cell Collection, Manassas, VA, USA). All reagents were analytically pure and proper for cell culture use. Nicotine ((−)-1-Methyl-2-(3-pyridyl) pyrrolidine; purity ≥ 99%) was acquired from Sigma-Aldrich, Merck KgaA (Darmstadt, Germany).