Early passage hAMSCs and BTIC cultures were used and authenticated by Johns Hopkins Genetic Resources Core Facility. hAMSCs (Invitrogen, R7788-115) were cultured in MesenPRO complete media (1% Antibiotic/Antimycotic (Invitrogen, 15240-062), 1% Glutamax (GIBCO, 35050-061), 1 vial of MesenPRO RS growth supplement (GIBCO, 12748-018), and MesenPRO RS basal media (GIBCO, 12747-010)). Human BTIC cultures (276 and 612) were obtained from intraoperative tissue (as approved by Johns Hopkins Institutional Review Board) and cultured in laminin-coated flasks (Sigma, L2020, 1 μg/cm2) with stem cell media (30 (link)). As previously validated and shown by our group, the human BTIC cultures are able to form oncospheres, are multipotential, and form tumors when implanted into animal models (30 (link)). To evaluate the tumorigenic capacity of BTICs in vivo, BTIC line 276 was injected intracranially in mice by our group resulting in the formation of solid tumors, while 612 formed diffuse tumors (30 (link), 31 (link)). The molecular subtype of BTIC culture 276 is mesenchymal and 612 is proneural, which was determined using a metagene score based approach for subtype designation, assessing four mesenchymal and two proneural genes using a microfluidics based qPCR assay (32 (link)). Commercial U87 cells (ATCC, HTB-14) were cultured in DMEM media with 10% FBS.
Hamsc
Manufactured by Thermo Fisher Scientific
Sourced in United States
HAMSCs are a type of laboratory equipment designed to provide controlled environments for cell culture applications. They offer temperature, humidity, and gas composition regulation to support the growth and maintenance of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Mesenpro rstm medium, by Thermo Fisher Scientific (2 mentions)
Complete hepc growth medium, by Celprogen (2 mentions)
Hbmsc, by Thermo Fisher Scientific (2 mentions)
Stempro accutase cell dissociation reagent, by Thermo Fisher Scientific (2 mentions)
L2020, by Merck Group (1 mentions)
Mesenpro rs basal media, by Thermo Fisher Scientific (1 mentions)
Mesenpro rs growth supplement, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
5 protocols using hamsc
1
Characterization of Brain Tumor Initiating Cells
2
Expansion of Human Adipose-Derived Mesenchymal Stem Cells
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Three kinds of hAMSCs were purchased from Invitrogen (Carlsbad, CA, USA), ATCC (Manassas, VA, USA), and Thermo Fisher Scientific, Inc. (Waltham, MA, USA), respectively. The cryopreserved cells were thawed at 37°C and then immediately cultured in MesenPRO RSTM medium (Gibco, Carlsbad, CA, USA). The cells were then expanded using MesenPRO RSTM medium to 5 passages. The medium was changed every three days until the cells were 70% confluent, at which time they were passaged.
3
Expansion of Human Adipose-Derived Mesenchymal Stem Cells
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Three kinds of hAMSCs were purchased from Invitrogen (Carlsbad, CA, USA), ATCC (Manassas, VA, USA), and Thermo Fisher Scientific, Inc. (Waltham, MA, USA). These cryopreserved cells were thawed at 37 °C and immediately cultured in MesenPRO RSTM medium (Gibco, Carlsbad, CA, USA). These cells were then expanded using the MesenPRO RSTM medium through five passages. The medium was changed every three days until cells were 70% confluent, at which time they were passaged.
4
Expansion and Maintenance of Human Stem Cells
5
Expansion and Maintenance of Human Stem Cells
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Human endothelial progenitor cells (hEPC) were purchased from Celprogen (San Pedro, Cat. No.: 37089-01) and were expanded on T75 flasks pre-coated with the extracellular matrix for hEPC expansion (Celprogen, Cat. No.: E36053-05-T75) in complete hEPC growth medium (Celprogen, Cat. No.: M36053-05ES). Human bone marrow-derived mesenchymal stem cells (hBMSC, Gibco, Cat. No.: A15652) and human adipose-derived mesenchymal stem cells (hAMSC, Gibco, Cat. No.: PCS-500-011) were maintained in a mesenchymal stem cell medium (MSCM) (ScienCell, Cat. No.: 7501). Human neural stem cells (hNSC, NR1), initially isolated from fetal cortical brain tissue at 13.5 weeks gestation (M031 clone) and derived from the embryonic stem cell line H9, were cultured in the same condition as the hiPSC-derived NSC. Human hematopoietic stem cells (hHSC) were purchased from ATCC (Cat. No.: PCS-800-012) and used directly for experiments without subculturing. Medium was changed every 2–3 days. Cells were passaged when the confluency reached approximately 80%. 0.25% trypsin-EDTA (disodium ethylenediaminetetraacetic acid) was used to passage transformed microglia, hBMSC, and hAMSC. 0.05% trypsin-EDTA was used to split astrocytes, BMEC, pericyte, hEPC, and hNSC. hNPC was passaged using StemPro™ Accutase™ Cell Dissociation Reagent (Gibco, Cat. No.: A1110501).
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