Ab13970

Manufactured by Synaptic Systems

Ab13970 is a laboratory equipment product. It is a core component used in scientific research applications.

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Lab products found in correlation

Ab53554, by Abcam (1 mentions) Ab6142, by Abcam (1 mentions) Ab182451, by Abcam (1 mentions) Nf κb p65 d14e12 xp rabbit mab, by Cell Signaling Technology (1 mentions) Neuronal nuclei (neun), by Synaptic Systems (1 mentions) Camkii α 6g9 mouse monoclonal antibody mab, by Cell Signaling Technology (1 mentions) Leica sp8 confocal microscope, by Leica camera (1 mentions) Las x software, by Leica Microsystems (1 mentions)

2 protocols using ab13970

Immunohistochemistry for Neural Markers

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The following antibodies were used: anti–phospho-histone H2A.X (Ser¹³⁹) antibody, clone JBW301, EMD Millipore, 05-636, 1:500; NeuN, Synaptic Systems, 266 004, 1:1000; CaMKII-α (6G9) mouse monoclonal antibody (mAb), Cell Signaling Technology, 50049S, 1:200; NEUROD1 polyclonal antibody, Proteintech, 12081-1-AP, 1:200; anti-GFP antibody, Abcam, ab13970, 1:500; Iba1, Synaptic Systems, 234 004, 1:1000; anti-GFAP antibody, Abcam, ab53554, 1:500; recombinant anti-Olig2 antibody (EPR2673), Abcam, ab109186, 1:500; RFP antibody preadsorbed, Fisher Scientific, 600-401-379, 1:200; NFκB p65 (D14E12) XP rabbit mAb, Cell Signaling Technology, 8242S, 1:500; NFκB p65 polyclonal antibody, Invitrogen, 51-0500, 1:100; anti–MHC class II (I-A/I-E), clone M5/114, EMD Millipore, MABF33, 1:500; anti-nestin antibody (rat 401), Abcam, ab6142, 1:1000; anti-C1q antibody (4.8), Abcam, ab182451, 1:500; synapsin 2 antibody (guinea pig), Synaptic Systems, 106 004, 1:500; VGlut1 (rabbit), Synaptic Systems, 135 303, 1:500; purified anti-mouse/rat β-amyloid antibody, BioLegend, 805801, 1:500; phospho-Tau (Thr¹⁸¹) (D9F4G) rabbit mAb, Cell Signaling Technology, 12885S, 1:500.

Welch G.M., Boix C.A., Schmauch E., Davila-Velderrain J., Victor M.B., Dileep V., Bozzelli P.L., Su Q., Cheng J.D., Lee A., Leary N.S., Pfenning A.R., Kellis M, & Tsai L.H. (2022). Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration. Science Advances, 8(39), eabo4662.

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3D Retinal Neuron Reconstruction

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For 3D reconstruction of confocal images from rat retina, the sections were stained with anti-NeuN (1:400, Abcam ab13970) and anti-MMP9 (1:400, Synaptic Systems 147,011). The confocal Z stack images were captured every 1 μm consecutively on a Leica SP8 confocal microscope (Leica, Tokyo, Japan) with oil objective (HC PL APO CS2 × 63/1.40). The Z stack images were simulated to 3D movies using LAS X software (Leica Microsystems).

Zhang K., Ma R., Feng L., Liu P., Cai S., Tong C, & Zheng J. (2022). Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn2+-dependent matrix metalloproteinase 9 during the early development. BMC Neuroscience, 23, 66.

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