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Azd6244

Manufactured by Cayman Chemical
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AZD6244 is a potent and selective inhibitor of the mitogen-activated protein kinase (MAPK) kinase 1/2 (MEK1/2) enzyme. It disrupts the MAPK signaling pathway, which is involved in cellular processes such as proliferation, differentiation, and survival. AZD6244 has been widely used in scientific research to study the role of the MAPK pathway in various biological systems.

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Lab products found in correlation

Ruthenium red, by Merck Group (2 mentions) Alpha amanitin, by Merck Group (2 mentions) Bapta am, by Merck Group (2 mentions) U0126, by Merck Group (2 mentions) A23187, by Merck Group (2 mentions) Thapsigargin, by Merck Group (2 mentions) Ly294002, by Merck Group (2 mentions) Stepone software program, by Thermo Fisher Scientific (1 mentions) Rn00580641 m1, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Torin 1, by Cayman Chemical (1 mentions) Rapamycin, by Enzo Life Sciences (1 mentions) Phospho akt s473, by Cell Signaling Technology (1 mentions) Cox 2, by Cell Signaling Technology (1 mentions) Phospho p65 ser536, by Cell Signaling Technology (1 mentions) Phospho erk1 2t202 y204, by Cell Signaling Technology (1 mentions) Parthenolide, by Cayman Chemical (1 mentions) Tuberin, by Cell Signaling Technology (1 mentions) β actin, by EASYBIO (1 mentions)

6 protocols using azd6244

1

Pharmacological Modulation of Calcium Signaling

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The following drugs were used in experiments: 10 μM gadolinium III chloride (Gd3+) (for both zebrafish and cell culture) to block Piezo1, and 30μM Ruthenium Red or 10μM BAPTA-AM were used to inhibit calcium (all from Sigma). 10 μM Roscovitine was used to block CDK1. 5μM alpha-amanitin (Sigma) was used to inhibit RNA polymerase II and III. 1μM AZD6244 (Cayman Chemical) or 1μM U0126 (Sigma) were used to inhibit MEK1 and 2. 1μM A23187 or 5μM Thapsigargin (both from Sigma) were used to induce calcium influx.
Gudipaty S., Lindblom J., Loftus P., Redd M., Edes K., Davey C., Krishnegowda V, & Rosenblatt J. (2017). Mechanical stretch triggers rapid epithelial cell division through Piezo1. Nature, 543(7643), 118-121.
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2

Pharmacological Modulation of Calcium Signaling

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The following drugs were used in experiments: 10 μM gadolinium III chloride (Gd3+) (for both zebrafish and cell culture) to block Piezo1, and 30μM Ruthenium Red or 10μM BAPTA-AM were used to inhibit calcium (all from Sigma). 10 μM Roscovitine was used to block CDK1. 5μM alpha-amanitin (Sigma) was used to inhibit RNA polymerase II and III. 1μM AZD6244 (Cayman Chemical) or 1μM U0126 (Sigma) were used to inhibit MEK1 and 2. 1μM A23187 or 5μM Thapsigargin (both from Sigma) were used to induce calcium influx.
Gudipaty S., Lindblom J., Loftus P., Redd M., Edes K., Davey C., Krishnegowda V, & Rosenblatt J. (2017). Mechanical stretch triggers rapid epithelial cell division through Piezo1. Nature, 543(7643), 118-121.
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3

Quantitative Analysis of Cardiac Gene Expression

Cited 2 times
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After 6 h of stimulation by the indicated hormone (10 nM, 500 nM or 50 μM of T1AM, 100 nM of ET-1, and 20 μM of PE), each dish was snap frozen. Where indicated, NRCM were first pretreated with 500 nM of AZD6244 (Cayman Chemical) or 10 μM of H-89 30 min prior to the indicated hormone stimulation. Total RNA was extracted from the frozen cells using TRIzol reagent (Invitrogen) and a quantitative real-time PCR was performed using a StepOnePlus Real-time PCR System and the StepOne Software program (Applied Biosystems), as described previously15 (link),37 (link). The real-time PCR protocol consisted of one cycle at 95 °C for 20 s followed by 40 cycles at 95 °C for 1 s and 60 °C for 20 s using the primers for NPPB (Applied Biosystems, Rn00580641_m1) and GAPDH (Applied Biosystems, Rn01775763_g1). The transcriptional levels were determined using the ΔΔCt method with normalization to GAPDH15 (link).
Takahashi H., Nagoshi T., Kimura H., Tanaka Y., Yasutake R., Oi Y., Yoshii A., Tanaka T.D., Kashiwagi Y, & Yoshimura M. (2022). Substantial impact of 3-iodothyronamine (T1AM) on the regulations of fluorescent thermoprobe-measured cellular temperature and natriuretic peptide expression in cardiomyocytes. Scientific Reports, 12, 12740.
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4

Signaling Pathways in Cell Apoptosis

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Rapamycin (ENZO), Torin1 (cayman), AZD6244 (cayman), Parthenolide (cayman), Bortezomib (cayman), prostaglandin E2 (MCE). Antibodies used were Anti‐glutaredoxin‐1 (Abcam, catalogue ab45953); Bim (ENZO, catalogue ADI‐AAP‐330‐E); β‐actin (EASYBIO, catalogue BE0037); Anti‐Glutathione (Santa Cruz, catalogue sc‐52399); p65 (Santa Cruz, catalogue sc‐8008); Bim (Santa Cruz, catalogue sc‐374358). The antibodies below are from Cell Signaling Technology: Tuberin (catalogue 4308); phospho‐Akt (S473) (catalogue 9271); phospho‐S6(Ser235/236) (catalogue 2211); Raptor (catalogue 2280); Rictor (catalogue 2114); cleaved caspase3 (catalogue 9661); cleaved PARP (catalogue 9546); Cox2 (catalogue 12282); phospho‐p65(Ser536) (catalogue 3033); phospho‐ERK1/2(T202/Y204) (catalogue 9101).
Feng Y., Li T., Li Y., Lin Z., Han X., Pei X., Zhang Y., Li F., Yang J., Shao D, & Li C. (2023). Glutaredoxin‐1 promotes lymphangioleiomyomatosis progression through inhibiting Bim‐mediated apoptosis via COX2/PGE2/ERK pathway. Clinical and Translational Medicine, 13(7), e1333.
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5

Characterization of Apoptosis Signaling

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Recombinant human super-Fas-L was obtained from Enzo Life Sciences (L¨orrach, Germany) and recombinant human mega-Fas-L from AdipoGen (San Diego, CA, USA). z-VAD.fmk (pan-caspase inhibitor), LY294002 (PI3K inhibitor), and SP600125 (JNK inhibitor) were obtained from Merck Biosciences (Darmstadt, Germany) and AZD6244 (ERK-1/2 inhibitor) was obtained from Cayman Chemical (Michigan, USA).
The following antibodies were used for flow cytometry stainings: anti-human CD95 (APO-1/Fas) APC and APC mouse IgG1 k isotype control (eBioscience San Diego, CA), anti-human CD31 APC and mouse IgG1 isotype control APC, anti-human CD34 PE, and mouse IgG1 k Isotype control PE (PeproThec London, UK), anti-human CD29 Alexa Fluor 488 and Alexa Fluor 488 mouse IgG1 k isotype control, anti-human CD105 PerCP/Cy5.5 and PerCP/Cy5.5 mouse IgG1 k Isotype control, anti-human 73 PE/Cy7 and PE/Cy7 mouse IgG1 k isotype control (BioLegend (San Diego, CA), anti-human CD45 BD Horizon BV650 and BV650 mouse IgG1, k isotype control BD Biosciences (San Jose, CA).
Solodeev I., Meilik B., Volovitz I., Sela M., Manheim S., Yarkoni S., Zipori D., Gur E, & Shani N. (2018). Fas-L promotes the stem cell potency of adipose-derived mesenchymal cells. Cell Death & Disease, 9(6), 695.
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6

Cell Culture and Compound Treatment

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DLD-1, SW480, HCT-116, and RPE-1 cells were maintained in Dulbecco’s modified Eagle’s medium (Bio-west) supplemented with 10% fetal bovine serum (Gibco) and an antibiotic-antimycotic mixture (Gibco). Cells at 70 to 80% confluence were treated with Compounds or dimethyl sulfoxide vehicle. PGE2, CAY10598, GW627368X, and AZD6244(Selumetinib) were from Cayman Chemical. FR-180204 was from TOCRIS Bioscience. LY294002, IKK16, and Verteporfin were purchased from Sigma Aldrich.
Kim H.B., Kim M., Park Y.S., Park I., Kim T., Yang S.Y., Cho C.J., Hwang D., Jung J.H., Markowitz S.D., Hwang S.W., Yang S.K., Lim D.S, & Myung S.J. (2016). Prostaglandin E2 Activates YAP and a Positive-signaling Loop to Promote Colon Regeneration Following Colitis but Also Carcinogenesis in Mice. Gastroenterology, 152(3), 616-630.
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