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Pan actin

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Pan-actin is a purified actin isoform. It is a globular protein that forms long polymers, known as actin filaments, which are a major component of the cytoskeleton in eukaryotic cells.

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Lab products found in correlation

Alexa488 anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) β tubulin, by Merck Group (2 mentions) H3k4me2, by Cell Signaling Technology (2 mentions) Fmoc l histidine amino acids, by Merck Group (2 mentions) Setd3, by Abcam (2 mentions) H3k36me2, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Anti-pan-actin (3 citations)

2 protocols using pan actin

1

Biotinylated Peptide Synthesis and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biotinylated peptides were synthesized at the UNC High-Throughput
Peptide Synthesis and Array Facility (Chapel Hill, NC)22 (link). Fmoc-L-histidine amino acids with
either (1-me) or (3-me) modifications (Sigma-Aldrich) were used in the synthesis
of these peptides and synthesized peptides were purified by HPLC (>92%
purity). Antibodies used in this study: SETD3 (Abcam), pan-actin (Cytoskeleton),
beta-tubulin (Millipore), H3K4me2 (Cell Signaling), H3K36me2 (Cell Signaling),
H73(3-me) see below, GFP (Invitrogen), Alexa488 anti-rabbit antibody (Life
Technologies), anti-rabbit and anti-mouse peroxidase conjugated antibodies
(Jackson Immunoresearch). Western blots were visualized by chemiluminescence (GE
Healthcare).
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
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2

Biotinylated Peptide Synthesis and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biotinylated peptides were synthesized at the UNC High-Throughput
Peptide Synthesis and Array Facility (Chapel Hill, NC)22 (link). Fmoc-L-histidine amino acids with
either (1-me) or (3-me) modifications (Sigma-Aldrich) were used in the synthesis
of these peptides and synthesized peptides were purified by HPLC (>92%
purity). Antibodies used in this study: SETD3 (Abcam), pan-actin (Cytoskeleton),
beta-tubulin (Millipore), H3K4me2 (Cell Signaling), H3K36me2 (Cell Signaling),
H73(3-me) see below, GFP (Invitrogen), Alexa488 anti-rabbit antibody (Life
Technologies), anti-rabbit and anti-mouse peroxidase conjugated antibodies
(Jackson Immunoresearch). Western blots were visualized by chemiluminescence (GE
Healthcare).
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
+ Open protocol
+ Expand

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