Avance 500

Manufactured by Bruker

Sourced in Germany, United States, Switzerland, France, China

The Avance 500 is a high-performance nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It provides advanced analytical capabilities for chemical and biological research applications.

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Lab products found in correlation

Avance 400, by Bruker (32 mentions) Avance 300, by Bruker (23 mentions) Avance 600, by Bruker (17 mentions) Topspin, by Bruker (6 mentions) Avance 800, by Bruker (5 mentions) Drx 400, by Bruker (5 mentions) Topspin 2, by Bruker (4 mentions) Avance 250, by Bruker (4 mentions) Silica gel 60 f254, by Merck Group (4 mentions) P 2000 polarimeter, by Jasco (4 mentions) Sephadex lh 20, by GE Healthcare (4 mentions) Silica gel 60, by Merck Group (4 mentions) Ltq orbitrap xl, by Thermo Fisher Scientific (3 mentions) Kieselgel 60 f254, by Merck Group (3 mentions) Uv 2501pc spectrophotometer, by Shimadzu (3 mentions) P 2000, by Jasco (3 mentions) Ir 3600 ft ir spectrometer, by Shimadzu (3 mentions) Topspin software, by Bruker (3 mentions) Microtof q instrument, by Bruker (3 mentions) Avance 700, by Bruker (3 mentions)

Close spelling variants of this product

Avance III HD 500 (68 citations) Avance III HD Ascend-500 (2 citations) AVANCE DMX 500 NMR spectrometer (7 citations) Avance 500 FT-NMR spectrometer (4 citations) AVANCE 500) UltraShield-NMR spectrometer (3 citations) Avance III 500 spectrometer (108 citations) Avance 500 or 600 spectrometers (3 citations) AVANCE III 500 NMR instrument (2 citations) Avance Spectrospin-500 spectrometer (2 citations) AVANCE AV-500 (11 citations)

319 protocols using avance 500

Characterization of Organic Compounds

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¹H NMR spectra were recorded
on a Bruker AVANCE 500, 600, or 800 (at 500/600/800 MHz). ¹³C NMR spectra were recorded on a Bruker AVANCE 500, 600, or 800 (at
125/150/200 MHz). Shimadzu LC-MS2020 was used to record ESI-MS data.
Agilent 1290 G6460A Q-TOF was used to perform high-resolution mass
spectrometry (HRMS). Size-exclusion chromatography was carried out
by using a Bio-Gel P-2, P-4, or P6 column. The absorbance was recorded
and analyzed at 450 nm on BioTek Synergy 2.

Zhang L., Liu Y., Xu Z., Hao T., Wang P.G., Zhao W, & Li T. (2022). Design and Synthesis of Neutralizable Fondaparinux. JACS Au, 2(12), 2791-2799.

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Synthesis of Myo-Inositol Monoacetal

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The monoacetal derivative of myo-inositol (see Scheme 1) was synthesized by reacting myo-inositol with 1,1-dimethoxycyclohexane in the presence of p-toluenesulfonic acid. All the chemicals used were purchased from Sigma-Aldrich or Alfa Aesar. The progress of the reaction was monitored using analytical thin-layer chromatography (TLC) on silica-gel plates (Silica Gel 60 F254 from Merck). Compound (1)Á2H 2 O was characterized by an analysis of the NMR spectra, which were recorded in DMSO-d 6 . 1 H NMR spectra were obtained on a Bruker Avance 500 (500 MHz) and 13 C NMR spectra were obtained on a Bruker Avance 500 (126 MHz); chemical shifts are expressed in (ppm) using the solvent peak as an internal standard. The multiplicity of the resonance peaks is indicated as singlet (s), doublet (d), triplet (t), quartet (q) or multiplet (m). The 13 C signals were assigned with the aid of the attached proton test (APT) and the J values are in Hertz.

Purushothaman G., Juvale K., Kirubakaran S., Vemula P.K, & Thiruvenkatam V. (2017). Water-mediated intermolecular interactions in 1,2-O-cyclohexylidene-myo-inositol: a quantitative analysis. Acta crystallographica. Section C, Structural chemistry, 73(Pt 1).

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Comprehensive NMR and IR Analysis

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¹H NMR spectra were recorded at
25 °C on either a Bruker Avance 400 (400 MHz) or Bruker Avance
500 (500 MHz) instrument and processed using MestReNova NMR processing
software. Chemical shifts (δ) are reported in parts per million
(ppm) downfield from tetramethylsilane and referenced to the residual
protium signal in the NMR solvents. Data are reported as follows:
chemical shift, multiplicity (s = singlet, d = doublet, t = triplet,
m = multiplet, and q = quartet), coupling constant (J) in Hertz (Hz), and integration. ¹³C spectra were recorded
at 25 °C on a Bruker Avance 500 instrument operating at 126 MHz.
Chemical shifts (δ) are reported in ppm downfield from tetramethylsilane
and referenced (except in D₂O) to the primary carbon resonance
in the NMR solvent. FT-IR spectra were recorded on a Shimadzu FT-IR
spectrophotometer. High-resolution mass spectra (HRMS) were acquired
at the University of Buffalo Chemistry Department Mass Spectrometry
Facility, Buffalo, NY.

Ofori L.O., Hilimire T.A., Bennett R.P., Brown NW J.r., Smith H.C, & Miller B.L. (2014). High-Affinity Recognition of HIV-1 Frameshift-Stimulating RNA Alters Frameshifting in Vitro and Interferes with HIV-1 Infectivity. Journal of Medicinal Chemistry, 57(3), 723-732.

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Optimized Organic Synthesis Techniques

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Chemicals were purchased from Sigma-Aldrich
or Fluorochem and were used without purification. Solvents were purchased
from VWR Chemicals (CH₂Cl₂) or Sigma-Aldrich
[toluene, dioxane, and dimethyl sulfoxide (DMSO)] and used without
purification unless stated otherwise. Dry solvents were dried over
an inert PS-MD-5 solvent purification system, equipped with an activated
alumina/copper wire column. Microwave reactions were performed in
a closed vessel in a Biotage Initiator, measuring temperature by IR. ¹H NMR measurements were acquired on a Bruker AVANCE 300 (300.13
MHz) or Bruker AVANCE 500 (500.23 MHz) spectrometer. ¹³C NMR measurements were acquired on a Bruker AVANCE 500 (125.78 MHz)
spectrometer. Chemical shifts are reported in parts per million downfield
of tetramethylsilane and are corrected according to the solvent. Mass
analysis was performed using a Bruker MicrOTOF-Q instrument on a positive
ion polarity mode for ESI (electrospray ionization). Capillary charge:
4000 V. Melting points were measured using a Büchi M-565 melting
point apparatus. SiO₂ column chromatography was performed
using Merck silica gel C60 (particle size 40–60 μm).
Thin-layer chromatography (TLC) was performed on Merck silica gel
C60 F254 plates (silica coat on the aluminum support). All isolated
yields are corrected for impurities (if present).

Collet J.W., van der Nol E.A., Roose T.R., Maes B.U., Ruijter E, & Orru R.V. (2020). Synthesis of Quinazolin-4-ones by Copper-Catalyzed Isocyanide Insertion. The Journal of Organic Chemistry, 85(11), 7378-7385.

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General Organic Chemistry Procedures

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Chemicals were purchased from Sigma-Aldrich
or Fluorochem and were used without purification. Solvents were purchased
from VWR Chemicals (CH₂Cl₂) or Sigma-Aldrich
(toluene, dioxane, DMSO) and used without purification, unless stated
otherwise. Dry solvents were dried over an inert PS-MD-5 solvent purification
system, equipped with an activated alumina/copper wire column. ¹H NMR measurements were acquired on a Bruker Avance 300 (300.13
MHz) or Bruker Avance 500 (500.23 MHz) spectrometer. ¹³C NMR measurements were acquired on a Bruker Avance 500 (125.78 MHz)
spectrometer. Chemical shifts are reported in parts per million downfield
of tetramethylsilane and are corrected according to solvent. Mass
analysis was performed using a Bruker MicrOTOF-Q instrument on a positive
ion polarity mode for ESI (electrospray ionization). Capillary charge:
4000 V. Melting points were measured using a Büchi M-565 melting
point apparatus. SiO₂ column chromatography was performed
using Merck silica gel C60 (particle size 40–60 μm).
TLC chromatography was performed on Merck silica gel C60 F254 plates
(silica coat on aluminum support). All isolated yields are corrected
for present impurities (if present).

Collet J.W., Ackermans K., Lambregts J., Maes B.U., Orru R.V, & Ruijter E. (2017). Modular Three-Component Synthesis of 4-Aminoquinolines via an Imidoylative Sonogashira/Cyclization Cascade. The Journal of Organic Chemistry, 83(2), 854-861.

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Analytical Characterization of Lomofungin

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Analysis of lomofungin (Enzo Life Sciences, BML-A245–0050) was performed on a Shimadzu LC2010 HPLC equipped with a C18 reverse-phase column. ¹H NMR spectra were recorded at 25°C on a Bruker Avance 400 (400 MHz) or Bruker Avance 500 (500 MHz) instrument. Chemical shifts (δ) are reported in parts per million (ppm) downfield from tetramethylsilane and referenced to the residual protium signal in the nuclear magnetic resonance (NMR) solvent (CDCl₃, δ = 7.26). Data are reported as chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, m = multiplet), integration, and coupling constant (J) in Hertz (Hz). ¹³C NMR spectra were likewise recorded at 25°C on a Bruker Avance 400 (100 MHz) or Bruker Avance 500 (125 MHz). Chemical shifts (δ) are reported in parts per million (ppm) downfield from tetramethylsilane and referenced to carbon resonances in the NMR solvent. High-resolution mass spectra were acquired at the University of Buffalo mass spectrometry facility, Buffalo, NY, USA.

Hoskins J.W., Ofori L.O., Chen C.Z., Kumar A., Sobczak K., Nakamori M., Southall N., Patnaik S., Marugan J.J., Zheng W., Austin C.P., Disney M.D., Miller B.L, & Thornton C.A. (2014). Lomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects. Nucleic Acids Research, 42(10), 6591-6602.

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Spectroscopic Characterization of Curcumin and Chitosan Conjugates

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The FTIR spectra for CUR, CS, CCS, PEG and PCC1 were recorded on FTIR spectrometer (Nicolet, TM Nexus 470-ESP, Thermo Fisher Scientific, Waltham, MA, USA) using KBr pellets. The ¹H NMR spectra for CUR and PEG were recorded on a 500 MHz NMR spectrometer (BRUKER AVANCE-500, Bruker, Billerica, MA, USA) using DMSO-d6 solvent. The ¹H NMR spectra for CS, PCC1, PCC2 and PCC3 were recorded on the same NMR spectrometer using CD₃COOD/D₂O solvent (1%, v/v). CUR was dissolved in methanol to obtain 200 µg/mL solution and PCC conjugates and CS were dissolved in acetic acid (1%, v/v) to obtain 1 mg/mL (w/v) for each solution. Then, the absorbances of these solutions were scanned from 200 nm to 700 nm wavelengths using an UV-Visible spectrophotometer (Shimadzu UV-2550, Kyoto, Japan).

Chen Y., Wu D., Zhong W., Kuang S., Luo Q., Song L., He L., Feng X, & Tao X. (2018). Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone. Nanomaterials, 8(7), 486.

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NMR Characterization of Organic Compounds

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¹H (500.1 MHz) and ¹³C (125.6 MHz) NMR spectra were recorded at room temperature on a Bruker 500 Avance III spectrometer equipped with a cryo probehead, or a Bruker Avance 500 (Bruker Biospin Co., Karlsruhe, Germany) spectrometer. Amounts of approximately 0.8–5 mg of the compounds were dissolved in 0.1 mL of methanol-d₄ and transferred to 2.5 mm Bruker MATCH NMR sample tube. Chemical shifts are given on the δ-scale and are referenced to the solvent (methanol- d₄: δC = 49.1 and δH = 3.31 ppm). Pulse programs of all experiments (¹H, ¹³C, DEPTQ, DEPT-135, sel-TOCSY (mixing time: 120 ms), sel-ROE (300 ms), gradient-selected (gs) ¹H,¹H-COSY, edited gs-HSQC, gs-HMBC (optimized for 10 Hz), ROESY (350 ms) were taken from the Bruker software library. For 1D measurement, 64 K data points were used to yield the FID. For 2D measurements, sweep width in F2 was 4000 Hz; all data points (t2 × t1) were acquired with 2 K × 256. For F1, linear prediction was applied to enhance the resolution. Most ¹H assignments were accomplished using general knowledge of chemical shift dispersion with the aid of the proton-proton coupling pattern (¹H NMR spectra). The NMR signals of the products were assigned by comprehensive one- and two-dimensional NMR methods using widely accepted strategies15 16 .

Hunyadi A., Herke I., Lengyel K., Báthori M., Kele Z., Simon A., Tóth G, & Szendrei K. (2016). Ecdysteroid-containing food supplements from Cyanotis arachnoidea on the European market: evidence for spinach product counterfeiting. Scientific Reports, 6, 37322.

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Schlenk Techniques for Air- and Moisture-Sensitive Compounds

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All the manipulations of the air- and moisture-sensitive compounds were carried out using standard Schlenk techniques under an argon atmosphere and the solvents were purified and degassed following standard procedures. All the reagents were purchased from commercial chemical suppliers (Acros (Illkirch, France), Alfa Aesar (Lancashire, UK), and TCI Europe (Paris, France)) and used without further purification. ¹H and ¹³C nuclear magnetic resonance (NMR) spectra were recorded on a Brucker AVANCE 300 or Bruker AVANCE 500 spectrometer (Bruker, Wissembourg, France) using the residual solvent peak as a reference (CDCl₃: δ_H = 7.26 ppm; δ_C = 77.16 ppm) at 295 K. The HMQC ¹H-¹⁹⁵Pt spectra were recorded on a Bruker AVANCE 600 spectrometer using the residual solvent peak as reference for the ¹H calibration and an external reference for the ¹⁹⁵Pt (H₂PtCl₆ in D₂O: δ_Pt = 0 ppm) at the Institut de Chimie NMR Facility of the University of Strasbourg. Positive mode electrospray ionization mass spectra (ESI-HRMS) analyses were carried out on microTOF, Bruker Daltonics (Bruker, Wissembourg, France).
All the syntheses and characterizations are available in the Supplementary Materials.

Bouché M., Vincent B., Achard T, & Bellemin-Laponnaz S. (2020). N-Heterocyclic Carbene Platinum(IV) as Metallodrug Candidates: Synthesis and 195Pt NMR Chemical Shift Trend. Molecules, 25(14), 3148.

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Synthesis and Characterization of Pd(II) and Pt(II) Complexes

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Na₂PdCl₄ and K₂PtCl₄ were purchased from Strem and used as received. 2,6-Bis(1-ethyl-benzimidazol-2′-yl)pyridine (L^BZ),^{12 (link)} and 4′-(2-pyridyl)-2,2′:6′,2′′-terpyridine (L^PY)^{13 (link)} were synthesized according to the published procedures. Elemental micro-analysis was achieved with a Vario Micro Cube analyzer from Elementar Analysensysteme or an EA 3000 elemental analyser from HEKtech. The electrospray mass spectra were registered with a ThermoFisher Exactive Plus instrument with an Orbitrap mass analyzer at a resolution of R = 70.000 and a solvent flow rate of 50 μL min⁻¹. The electronic absorption spectra were recorded on a Specord 210 Plus spectrophotometer. The ATR IR spectra of the solid-state complexes were recorded on a Brucker Alpha-E instrument. The ¹H, ¹³C, ¹⁹F and ³¹P NMR spectra were recorded with Bruker-Avance 500 (¹H, 500.13 MHz; ¹³C{¹H}, 125.77 MHz; ¹⁹F, 470.59 MHz; ³¹P, 202.46 MHz) and Bruker-Avance 400 (¹H, 400.40 MHz; ¹³C{1H}, 100.68 MHz; ³¹P, 162.08 MHz) spectrometers. The assignment of NMR signals was performed with the aid of two-dimensional NMR methods, {¹H, ¹H} COS90 and {¹H, ¹³C} HSQC.

, & Mansour A.M. (2021). Pd(ii) and Pt(ii) complexes of tridentate ligands with selective toxicity against Cryptococcus neoformans and Candida albicans. RSC Advances, 11(63), 39748-39757.

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