GO sheets and GQDs were characterized using an atomic force microscopy (AFM; Park Systems, XE-70) in the tapping mode and a transmission electron microscopy (TEM; JEOL, JEM-2100F) operated at an accelerating voltage of 200 kV. TEM samples were prepared on lacey-carbon TEM grids (Ted Pella, Inc.). PL and QY measurements were carried out using a fluorescence spectrometer (Scinco, FS-2) and a UV-vis spectrometer (Analytik Jena, Specord200). In order to measure long-term stability, as-prepared GQDs were kept in solution state under ambient conditions over 12 months. Photographs of fluorescent GQD solutions and composite films and fibers were taken using a digital camera (Canon, EOS 600D) with a 356 nm UV lamp (Specroline, ENF-260C/FE). The fluorescent GQD fibers were observed using a fluorescence microscopy (Olympus, BX51/U-RFL-T) with a UV excitation filter (Olympus, U-MNU2). X-ray photoelectron spectroscopy (XPS) measurements were carried out using a spectrometer (Thermo Scientific, Theta probe) with monochromatic Al Kα radiation. Fourier-transform infrared spectroscopy (FT-IR; Thermo Scientific, Nicolet 6700) was conducted with KBr pellets. Raman spectroscopy (JASCO, NRS-3100) was performed with 514 nm laser excitation.
Specord 200
Manufactured by Analytik Jena
Sourced in Germany
The Specord 200 is a UV-Vis spectrophotometer designed for accurate and reliable absorbance measurements. It features a double-beam optical system and a wavelength range of 190 to 1100 nanometers. The Specord 200 provides precise and reproducible results for a variety of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Specord 200 double beam, by Analytik Jena (3 mentions)
Nicolet 6700, by Thermo Fisher Scientific (3 mentions)
Sb120 dt, by Ningbo Scientz Biotechnology (3 mentions)
Model fp114, by Thermo Fisher Scientific (2 mentions)
Ea3000, by Eurovector (2 mentions)
Origin 7, by OriginLab (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
U mnu2, by Olympus (1 mentions)
Nrs 3100, by Jasco (1 mentions)
Theta probe, by Thermo Fisher Scientific (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Xe 70, by Park Systems (1 mentions)
Metoprolol tartrate, by Tokyo Chemical Industry (1 mentions)
Propranolol hydrochloride, by Tokyo Chemical Industry (1 mentions)
Benzoyl peroxide, by Merck Group (1 mentions)
Ultimate 3000, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Ethylene glycol dimethacrylate, by Merck Group (1 mentions)
74 protocols using specord 200
1
Comprehensive Characterization of Graphene Quantum Dots
2
In Vitro Diclofenac Release Kinetics
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The release of DICL was accomplished through the immersion of the DICL-loaded hydrogels in 25 mL of selected media, i.e., 0.01 M HCl, water, unbuffered PBS solution, and 0.01 M NaOH. The release of diclofenac was monitored spectrophotometrically, using the UV–Vis spectrometer Specord 200 (Analytic Jena, Jena, Germany), via measuring the absorbance at λmax = 278 nm, which is characteristic of DICL [33 (link),34 (link),35 (link)]. The release experiments were carried out until a plateau was observed in the release curves, which was a sign that no more DICL would be able to be released from the tested hydrogels.
3
UV-Vis Spectrophotometry of Graphene Oxide
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Optical absorbance measurements were performed with a Specord 200 (AnalytikJena, Jena, Germany) UV-Vis Spectrophotometer. Data were collected in the whole UV-Vis range (190–1100 nm). The typical UV absorption peak of the GO appears around 230 nm.
4
Analytical Determination of Beta-Blockers
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(R)-(+)-atenolol (ATE), metoprolol tartrate (MET) and propranolol hydrochloride (PRO) were purchased from Tokyo Chemical Industry. ITA, ethylene glycol dimethacrylate (EGDMA), benzoyl peroxide (BPO), triethylamine (TEA) and trifluoroacetic acid (TFA) were purchased from Sigma Aldrich. HPLC grade acetonitrile and methanol were purchased from Fischer Scientific. Acetone, potassium bromide and acetic acid were purchased from Merck. Blood samples were provided by the Indonesian Red Cross. If not otherwise specified, all chemicals are analytical grade. The morphological evaluation analysis was carried out by JSM-6610LV JEOL Ltd. A UV-visible spectrophotometer (Analytic Jena, Specord 200) was used to detect the UV absorbance for constant association determination. Analyses of blood after extraction with the MI-SPE were performed using HPLC (Dionex Ultimate 3000 with UV detector) by isocratic elution, using a mixture of methanol/water containing 0.01% TEA (15:85) as the mobile phase and a column Merck C18G 125A (250 × 4.6 mm i.d.). The flow rate was 1 mL min−1, and the detection wavelength was set at 229 nm. The column was passed through with mobile phase until the baseline was obtained before detecting the samples.
5
Polymer Membrane Characterization and Analysis
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All chemicals used were of analytical grade, and they were used without further purification. All solutions were made using distilled water. Acetosal, ferric chloride (FeCl3), folin, ibuprofen, potassium iodide (KI), mercury chloride (HgCl2), sodium hydroxide (NaOH), sodium nitrite (NaNO2), and paracetamol were purchased from Merck. Sulphuric acid (H2SO4) and ethanol 96% were obtained from Emsure. Metampyrone was obtained from Medialabs. Ethyl acetate was obtained from Bratacho Chemistry. Poly(methyl methacrylate) (PMMA) was obtained from Aldrich Chemistry. The characteristic of polymer membrane was observed by Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX) (Jeol JSM-651OLA). The absorbance measurement was recorded by UV-Visible spectrophotometer (Analytic Jena Specord 200).
6
Spectrophotometric Analysis of Samples
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All spectrophotometric measurements were made using UV-Vis Spectrophotometer (Specord200, Analytikjena, Germany) attached to HP computer. All assays were performed with a 1cm path length using a pair of matched quartz cuvettes. The pH measurements were made with the aid of a pH meter (Metrohm, Switzerland).
7
Antioxidant Capacity Evaluation of L-SEO
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The L-SEO sample’s antioxidant capacity was evaluated using two spectrophotometric assays (DPPH assay and ABTS assay), as reported earlier [22 ]. A UV-VIS spectrophotometer Specord 200 (Analytik Jena, Jena, Germany) and a 10 mm quartz cuvette were used in the procedure described above. Briefly, for the DPPH assay, we mixed a 0.1 mL control sample with 3 mL of 0.2 mM ethanolic DPPH• solution. The absorbance was recorded after 60 min of reaction in the dark at λ = 517 nm. Positive controls containing 0.02–4 mM Trolox solutions were prepared. The data are expressed in mg Trolox/L and inhibition (%).
For the ABTS assay, we mixed 0.5 mL sample or control with 1 mL ABTS* solution, prepared 16 h before from ABTS reagent and 2.45 mM aqueous solution of sodium persulfate. The absorbance was recorded at λ = 734 nm, after 10 min of reaction time in the dark. Positive controls containing 0.02–1.0 mM Trolox solutions were prepared. The data are expressed in mmol TEAC/L (TEAC: Trolox Equivalent Antioxidant Capacities) and inhibition (%). All the experiments were performed in triplicate.
For the ABTS assay, we mixed 0.5 mL sample or control with 1 mL ABTS* solution, prepared 16 h before from ABTS reagent and 2.45 mM aqueous solution of sodium persulfate. The absorbance was recorded at λ = 734 nm, after 10 min of reaction time in the dark. Positive controls containing 0.02–1.0 mM Trolox solutions were prepared. The data are expressed in mmol TEAC/L (TEAC: Trolox Equivalent Antioxidant Capacities) and inhibition (%). All the experiments were performed in triplicate.
8
Photosynthetic Pigment Quantification
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Concentrations of the photosynthetic pigments were measured at 8 DAS of the experiment. The sample (5 fresh leaves per treatment per ecotype) was powdered with liquid nitrogen in the presence of magnesium hydroxide carbonate. Photosynthetic pigments were extracted from about 0.1 g of homogenized leaf tissue with absolute ice-cold acetone for 15 min. After centrifugation (14,000× g, 4 °C, 10 min), the supernatant was collected, and reextractions were performed under the same conditions until the tissue became colorless. Chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoids (Car) were determined spectrophotometrically (Specord 200, Analytic Jena, Jena, Germany) at 470, 644.8, and 661.6 nm in joined supernatants and chlorophyll a/b ratio was calculated [54 (link)]. Concentrations of photosynthetic pigments were calculated according to the determined dry mass of samples and expressed as mg/g of DW.
9
Phytochemical Screening of Herbal Medicines
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All chemicals used were of analytical grade, and they were used without further purification. All solutions were made using distilled water. Allopurinol was obtained from Nanjing Pharma Chemical Plant. 2-Naphthol, ammonium hydroxide 25%, hydrochloric acid 37%, bismuth subnitrate, ethanol 95%, ferric chloride, potassium hydroxide, potassium iodide, sodium hydroxide, potassium chlorate crystals, sodium nitrite, sodium nitroprusside, sodium sulfite anhydrous, silver nitrate, Dragendorff reagent, p-DAB, and Folin-Ciocalteu reagent were purchased from Merck. Alkaline fuchsin was purchased from Sigma Aldrich, and sample packaged herbs were purchased from traditional drug store around Jatinangor, Sumedang, West Java. Whatman No. 1, No. 4, and No. 6 and Whatman 1 chromatography qualitative-grade filter paper were purchased from GE Healthcare. The absorbance measurement was recorded by UV-Visible spectrophotometer Analytik Jena SPECORD 200 using a 1.0 cm quartz cell.
10
Soil Microbial Biomass Carbon Quantification
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Soil microbial biomass carbon (MBC) was measured by modified chloroform fumigation extraction method [24] . It was assayed by treating 10 g of fresh soil sample with 2 mL ethanol free chloroform in the soil sample and incubated for 24 hrs. In another set, soil was kept in similar condition except for chloroform treatment. After incubation, the lids of the container were opened to remove the chloroform vapors. 40 mL of 0.5 M K 2 SO 4 was added to it. The content was shaken for at least 1 hr. The suspension was filtered and the filtrate was measured at 280 nm in UV-Visible spectrophotometer (Specord 200, Analytik Jena, Germany).
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