Log In Sign up
The largest database of trusted experimental protocols

Gata3

Manufactured by Cell Marque
Visit Supplier
Sourced in United States

GATA3 is a protein marker that is used in immunohistochemistry (IHC) applications. It is a transcription factor that plays a role in the development and differentiation of certain cell types.

Automatically generated - may contain errors

Lab products found in correlation

E cadherin, by Agilent Technologies (1 mentions) Ck5 6, by Agilent Technologies (1 mentions) Ck7 ov tl 12 30, by Agilent Technologies (1 mentions) Nanozoomer s60 digital slide scanner, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

GATA3 (clone L50-823, (5 citations)

4 protocols using gata3

1

Immunohistochemical Staining of CK5/6 and GATA3

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were stained for CK5/6 (Clone: D5/16 B4; Dako /Agilent, Santa Clara, CA, USA) and GATA3 (Clone: L50-823; Cell Marque, Rocklin, CA, USA) as described before [19 (link)].
Koll F.J., Döring C., Olah C., Szarvas T., Köllermann J., Hoeh B., Chun F.K., Reis H, & Wild P.J. (2023). Optimizing identification of consensus molecular subtypes in muscle-invasive bladder cancer: a comparison of two sequencing methods and gene sets using FFPE specimens. BMC Cancer, 23, 504.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Tumor Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed tumors retrieved from the mice were embedded in wax and sections (5 μm) were prepared using standard methods and stained with eosin and hematoxylin. Sections were processed and stained individually for hematoxylin and eosin staining, ER (1:1, Roche), PR (1:1, Roche), HER2 (1:1, Roche), vimentin (1:400, Leica), E-cadherin (1:50, Dako), CK5/6 (1:50, Dako), GATA3 (1:100, Cell Marque), CK20 (1:5000, Biocare), and GCDFP (1:1000, Cell Marque) before observation by microscopy.
Kumar A., Tripathy M.K., Pasquereau S., Al Moussawi F., Abbas W., Coquard L., Khan K.A., Russo L., Algros M.P., Valmary-Degano S., Adotevi O., Morot-Bizot S, & Herbein G. (2018). The Human Cytomegalovirus Strain DB Activates Oncogenic Pathways in Mammary Epithelial Cells. EBioMedicine, 30, 167-183.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Patient tumor samples and tumors excised from patient-derived models were stained with IHC and Hematoxylin Eosin (HE). Primary antibodies used for IHC were as follows: HER2 (HER2/neu, Agilent Technologies, Glostrup, Denmark), CK7 (OV-TL12/30, Agilent Technologies, Glostrup, Denmark), CK20 (Ks20.8, Agilent Technologies, Glostrup, Denmark), and GATA3 (L50–823, Cell Marque, The Hague, Netherlands). Glass slide samples were digitized with a NanoZoomer S60 digital slide scanner (Hamamatsu Photonics, Hamamatsu, Japan).
Harada Y., Sato A., Nakamura H., Kai K., Kitamura S., Nakamura T., Kurihara Y., Ikeda S., Sueoka E., Kimura S, & Sueoka-Aragane N. (2023). Anti-cancer effect of afatinib, dual inhibitor of HER2 and EGFR, on novel mutation HER2 E401G in models of patient-derived cancer. BMC Cancer, 23, 77.
+ Open protocol
+ Expand
4

Immunohistochemical Staining of Tumor Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical stains performed at MD Anderson used 4 μm thick formalin-fixed paraffin-embedded tumor tissue sections and Leica system (Leica Biosystems, -Deer Park, IL, USA) for CAM5.2 keratin (BD Biosciences, Becton, NJ, USA), GATA3 (Cell Marque, Rocklin, CA, USA), pancytokeratin cocktail (keratin AE1/3, keratin 8/18, CAM5.2, and MNF116), p63 (Biocare Medical, Pacheco, CA, USA), and TRPS1 (EPR16171, Abcam, Cambridge, UK).
Zheng L., Luthra R., Alvarez H.A., San Lucas F.A., Duose D.Y., Wistuba I.I., Fuller G.N., Ballester L.Y., Roy-Chowdhuri S., Sweeney K.J., Rashid A., Yang R.K., Chen W., Liu A., Wu Y., Albarracin C., Patel K.P., Routbort M.J., Sahin A.A., Ding Q, & Chen H. (2023). Intragenic EGFR::EGFR.E1E8 Fusion (EGFRvIII) in 4331 Solid Tumors. Cancers, 16(1), 6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!