T2 cells were cultured in RPMI 1640 + Glutamax with 10% FBS, 1% penicillin/streptomycin. RPMI 6666 cells were grown in the same media and used as an additional A2+ cell line for negative-selection stages of phage panning.
Negative selection
Negative selection is a technique used to isolate specific cell populations from a heterogeneous mixture. It involves the removal of unwanted cells, allowing the desired cells to be obtained. The core function of negative selection is to selectively deplete target cells, resulting in the enrichment of the cell type of interest.
Lab products found in correlation
55 protocols using negative selection
Activation and Isolation of T Cells
T2 cells were cultured in RPMI 1640 + Glutamax with 10% FBS, 1% penicillin/streptomycin. RPMI 6666 cells were grown in the same media and used as an additional A2+ cell line for negative-selection stages of phage panning.
Naive T Cell Proliferation Assay
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Neutrophil-PBMC Co-culture Protocol
Neutrophil Enrichment from Casein-Induced Inflammation
Neutrophil Adhesion Assay: ICAM-1 and CXCL1
Isolation and Purification of Mouse Neutrophils
Quantifying HIV Reservoir in CD4+ T Cells
Isolation and Purification of PBMC
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