Rodent chow ca 1

All animal experiments were approved by the animal ethics committee of Kyushu University (A20-169-0, A21-271-0, and A22-013-0) and were conducted in compliance with the university guidelines and regulations for animal experimentation. For generation of Rpl3^–/– and Rpl3l^–/– mice, ribonucleoprotein was prepared by mixing the CRISPR RNA (crRNA) and transactivating crRNA (tracrRNA) with recombinant Cas9 protein (Integrated DNA Technologies) (Supplementary Data 9). Mouse zygotes of the C57BL/6 J strain were subjected to electroporation with each ribonucleoprotein complex. All mice were housed in the specific pathogen-free animal facility at Kyushu University in accordance with institutional guidelines under the following conditions: ambient temperature of 22 °C, 50% to 60% humidity, 12 h-dark/12 h-light cycle, and free access to water and rodent chow CA-1 (CLEA Japan). Male mice were used for all experiments, given that male mice are commonly used for cardiac analysis. Mice were euthanized in a CO₂ chamber for experiments.

All animal experiments were approved by the animal ethics committee of Kyushu University (A20-169-3) and were conducted in compliance with the university guidelines and regulations for animal experimentation. For generation of Kastor^–/–, Polluks^–/–, Kastor^–/–/Polluks^–/–, Vdac3^–/–, Kastor^FLAG/+, and Polluks^FLAG/+ mice, ribonucleoprotein (RNP) was prepared by mixing the CRISPR RNA (crRNA) and transactivating crRNA (tracrRNA) with recombinant Cas9 protein (Integrated DNA Technologies) (Supplementary Data 5). Single-stranded oligodeoxynucleotide (ssODN) homology repair templates were synthesized as 200-nt sequences (Supplementary Data 5). Mouse zygotes of the C57BL/6J strain were subjected to electroporation with each RNP with or without the corresponding ssODN. All mice were housed in the specific pathogen-free animal facility at Kyushu University in accordance with institutional guidelines under the following conditions: 22 °C ambient temperature, 50–60% humidity, 12 h dark/light cycle, and free access to water and rodent chow CA-1 (CLEA Japan). B6D2F1/Jcl (C57BL/6N Jcl × DBA/2N Jcl) female mice were used for the fertility test, and all other experiments were performed on the C57BL/6J background.