Xtt kit

The cell viability rate was determined using the trypan blue method. Hormone treated cells were exposed to trypan blue (Gibco, UK) after being trypsinized from a flask. The live and dead cells were counted using the Thoma Slide (Marienfeld, Germany).
The XTT Kit (Biological Industries, Israel) was used to determine the impacts of hormones on the proliferation of breast and prostate cancer cell lines. In brief, the cells were plated into 96-well plates (Greiner, Germany) and, after optimal hormone treatment, XTT was pipetted to all wells and cultured at 37°C with 5% CO₂ for 2 hours. The microplate spectrophotometer (Lab Systems, Finland)) was used to determine the absorbance at 450nm.
The XTT Kit (Biological Industries, Israel) was again used, this time to determine the impacts of hormones on the adhesion of breast and prostate cancer cell lines, and, as before, the cells were plated into 96-well plates (Greiner, Germany). After optimal hormone treatment, the wells were flushed three times with phosphate-buffered saline (PBS; Sigma, Saint Louis, USA). XTT was then pipetted to all wells and cultured at 37°C with 5% CO₂ for 2 hours, and the microplate spectrophotometer (Lab Systems, Finland)) was used to determine the absorbance at 450nm.

We estimated the viable cell numbers as described previously^{9 (link)}, using the XTT Kit (Biological Industries), which measures the reduction of a tetrazolium component by the mitochondria of viable cells. On the day of measurement, XTT was added to the culture media per the manufacturer’s instructions. Plates were then incubated at 37 °C for 3–5 h. Afterwards, the absorbance was read at 450 nm (reference absorbance, 630 nm).