Soy protein isolate (SPI, ~90% protein) and CGM (~62% protein) were supplied by Shansong Industrial Chinese Co. Ltd. and a grain processing refinery, Golshahd Co. Ltd., respectively. Alcalase 2.4 L from Bacillus licheniformis, with the activity of 2.4 Anson Units (AU)/g, and a density of 1.18 g/ml was purchased from Novozymes. 2,2 Diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis (3‐ethylbenzthiazoline‐6‐sulphonic acid) diammonium salt (ABTS), 4‐nitrophenyl α‐d‐glucopyranoside (PNPG), porcine pancreatic α‐amylase, rat intestinal α‐glucosidase, ACE (5 UN), hippuryl‐his‐leu (HHL), and ammonium salt of 1‐anilino‐8‐naphtalene‐sulphonic acid (ANS) were purchased from Sigma‐Aldrich. Soluble starch ACS reagent was purchased from Merck.
Diphenyl 1 picrylhydrazyl
Manufactured by Merck Group
Sourced in United States
Diphenyl-1-picrylhydrazyl is a stable free radical used as a reagent in various analytical and research applications. It is a deep violet crystalline solid with a characteristic absorption spectrum that can be used for the quantitative determination of antioxidant capacity. The compound is commonly employed in assays to evaluate the free radical scavenging ability of substances.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (3 mentions)
Acarbose, by Merck Group (2 mentions)
Trolox, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Diammonium salt, by Merck Group (1 mentions)
Alcalase 2.4 l from bacillus licheniformis, by Novozymes (1 mentions)
4 nitrophenyl α d glucopyranoside, by Merck Group (1 mentions)
Porcine pancreatic α amylase, by Merck Group (1 mentions)
Rat intestinal α glucosidase, by Merck Group (1 mentions)
2 2 azino bis, by Merck Group (1 mentions)
Anhydrous pyridine, by Merck Group (1 mentions)
Betain, by Merck Group (1 mentions)
Anthraquinone, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by Merck Group (1 mentions)
Avicel ph 101, by Merck Group (1 mentions)
Deuterated chloroform, by Merck Group (1 mentions)
Sodium salicylate, by Merck Group (1 mentions)
Cyclohexanol, by Merck Group (1 mentions)
7 protocols using diphenyl 1 picrylhydrazyl
1
Characterization of Antioxidant and Enzymatic Activities
2
Pretreatment of Poplar Wood for Bioenergy
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Steam pretreated poplar wood was kindly obtained from Forest Products Biotechnology/Bioenergy Group, Department of Wood Science (University of British Columbia, Vancouver, BC, Canada). Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), dimethyl sulfoxide (DMSO), cyclohexanol, anhydrous pyridine, deuterated chloroform, chromium(III) acetylacetonate, 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane (TMDP, Product No. 447536), betain, sodium salicylate, anthraquinone, commercial Kraft lignin (Product No. 370959) and microcrystalline cellulose (Avicel® PH-101) were purchased from Sigma-Aldrich. Other common chemicals and organic solvents with analytical grade were purchased from Kelong Chemical Regent Co., Ltd. (Chengdu, China).
3
In Vitro Bioactivity Screening Protocol
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All the chemicals and reagent used in this work were of laboratory and analytical grade. Diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,5-dinitrosalicylic acid (DNSA), α-amylase, acarbose, 4-nitrophenyl butyrate (p-NPB), lipase, orlistat, l-3,4-dihydroxyphenylalanine (L-DOPA), mushroom tyrosinase, kojic acid, N-succinyl-Ala-Ala-p-nitroanilide (AAAPVN), porcine pancreatic elastase, quercetin, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), acetylthiocholine iodide, butyrylcholine iodide, 5,5′-dithiobis-(2-nitrobenzoic acid) (DNTB), and galantamine were purchased from Sigma-Aldrich, USA.
4
Antioxidant and Carbohydrate Hydrolysis Assays
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Porcine α-amylase, α-glucosidase, diphenyl-1-picrylhydrazyl (DPPH), butylated hydroxytoluene (BHT), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), p-Nitrophenyl-α-glucopyranoside (PNPG) and acarbose were purchased from Sigma-Aldrich Co., St Louis, MO, USA, while soluble starch (extra pure) was obtained from HiMedia Laboratories (Mumbai, India). Other chemicals and reagents were of analytical grade. Water was double distilled.
5
Analytical Standards for Olive Oil Compounds
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n-Hexane, 0.5 N sodium methoxide, 14% boron trifluoride–methanol, Trolox, diphenyl-1-picryl-hydrazyl (DPPH), and Folin–Ciocalteu’s reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA); acetic acid, formic acid, methanol, acetonitrile (ACN), N,N-dimethylformamide (DMF), and tertbutylmethylether (TBME) from Sigma-Aldrich (Madrid, Spain); and sodium chloride (NaCl) and sodium carbonate (Na2CO3) from Panreac Química SLU (Castellar del Vallès, Spain). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).
Regarding the standards (≥90% purity), oleocanthal was purchased from Merck (Darmstadt, Germany), and oleacein, Oleuropein aglycone, and elenolic acid from Toronto Research Chemical Inc. (North York, ON, Canada). Oleuropein, ligstroside, pinoresinol, gallic acid, vanillic acid, caffeic acid, verbascoside, rutin, chlorophyll a, lutein, β-carotene, squalene, and (α)-tocopherol were acquired from Sigma-Aldrich. Apigenin, ferulic acid and p-coumaric were obtained from Fluka, and hydroxytyrosol from Extrasynthese (Genay, France). Methyl tridecanoate (C13:0) was used as a standard for the analysis of FAs and was acquired from Sigma-Aldrich.
Regarding the standards (≥90% purity), oleocanthal was purchased from Merck (Darmstadt, Germany), and oleacein, Oleuropein aglycone, and elenolic acid from Toronto Research Chemical Inc. (North York, ON, Canada). Oleuropein, ligstroside, pinoresinol, gallic acid, vanillic acid, caffeic acid, verbascoside, rutin, chlorophyll a, lutein, β-carotene, squalene, and (α)-tocopherol were acquired from Sigma-Aldrich. Apigenin, ferulic acid and p-coumaric were obtained from Fluka, and hydroxytyrosol from Extrasynthese (Genay, France). Methyl tridecanoate (C13:0) was used as a standard for the analysis of FAs and was acquired from Sigma-Aldrich.
6
Antioxidant Activity Determination Protocol
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All reagents were of analytical grade. Acetonitrile (ACN), tris(hydroxymethyl)aminomethane (Tris-HCl), 2-iodoacetamide (IAM), 1,4-dithiothreitol (DTT), trypsin from bovine pancreas (T1426, lyophilized powder, ≥10,000 units/mg protein), diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), azo 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH), Trolox, fluorescein were from Sigma-Aldrich (St. Louis, MO, USA). Mini-Protean apparatus, precision plus protein standards, Bradford reagent, and Coomassie Blue G-250 were purchased from Bio-Rad (Hercules, CA, USA).
7
Phytochemical Extraction and Analysis
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All the chemicals, reagents and solvents used were analytical grade: butanol, acetic acid, distillated water and ethanol for extraction (as bioethanol 70%).
Formic acid, gallic acid as well as the thin layer chromatography (TLC) plates (60 F254 silica gel plate on aluminum support) were purchased from Merck (Darmstadt, Germany).
Methanol (HPLC-MS grade), both flavonoid standards (quercetin and gallic acid), diphenyl-1-picrylhydrazyl (DPPH), Neu (diphenylboryloxyethylamine), Aluminium chlorid AlCl 3 2%, Sodium carbonate, Trolox from and Sodium carbonate were provided by Sigma-Aldrich (Steinheim, Germany). Folin-Ciocalteu Reagent was purchased from Fluka Analytical.
Formic acid, gallic acid as well as the thin layer chromatography (TLC) plates (60 F254 silica gel plate on aluminum support) were purchased from Merck (Darmstadt, Germany).
Methanol (HPLC-MS grade), both flavonoid standards (quercetin and gallic acid), diphenyl-1-picrylhydrazyl (DPPH), Neu (diphenylboryloxyethylamine), Aluminium chlorid AlCl 3 2%, Sodium carbonate, Trolox from and Sodium carbonate were provided by Sigma-Aldrich (Steinheim, Germany). Folin-Ciocalteu Reagent was purchased from Fluka Analytical.
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