Whole globes from each dosage group allocated for transmission electron microscopy were enucleated and fixed in 2% paraformaldehyde, 2% glutaraldehyde in 0.1 M sodium cacodylate buffer overnight at 4 °C. Following fixation, dissection of globes to obtain samples containing the cornea, iridocorneal angle, and anterior retina were completed and tissue post-fixed in 1% osmium tetroxide on ice. Following post-fixation, tissue was dehydrated in a methanol series (50–100%) and infused with acetonitrile. Tissue samples were then embedded in 100% Embed 812 (Electron Microscopy Sciences, Hatfield, PA, USA) and baked at 60 °C overnight. Embedded samples were processed by the Medical College of Wisconsin Electron Microscopy Core, where 70 nm sections were made using a PowerTome MT-XL ultramicrotome (RMC Boeckeler, Tucson, AZ, USA), placed on 200 mesh hexagonal grids (Electron Microscopy Science) and stained with uranyl acetate and lead citrate. Stained sections were imaged on a H-600 transmission electron microscope (Hitachi High Technologies, Schaumburg, IL, USA) at 2000× magnification.
H 600 transmission electron microscope
Manufactured by Hitachi
Sourced in Japan, United States, Sweden, China
The Hitachi H-600 transmission electron microscope is a high-performance imaging instrument designed for advanced materials analysis. It provides high-resolution, high-contrast imaging of sample structures at the nanometer scale. The H-600 utilizes an electron beam to illuminate and magnify samples, allowing for detailed visualization and characterization of a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
Zetasizer nano z, by Malvern Panalytical (3 mentions)
Ah 2 light microscope, by Olympus (3 mentions)
Coolsnap hq, by Teledyne (2 mentions)
C1 plus ex3 aom confocal system, by Nikon (2 mentions)
Ez c1, by Nikon (2 mentions)
Ultracut e, by Reichert Technologies (2 mentions)
Nis element, by Nikon (2 mentions)
Eclipse te300, by Nikon (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
Epon 812 resin, by Electron Microscopy Sciences (2 mentions)
Ecl western blotting kit, by Solarbio (2 mentions)
Total protein extraction kit, by Keygen Biotech (2 mentions)
200 mesh hexagonal grids, by Electron Microscopy Sciences (1 mentions)
Embed 812, by Electron Microscopy Sciences (1 mentions)
Avii 400, by Bruker (1 mentions)
F 4700, by Hitachi (1 mentions)
Uc7 ultramicrotome, by Leica (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Paraformaldehyde (pfa), by Solarbio (1 mentions)
Dynamic light scattering, by Malvern Panalytical (1 mentions)
87 protocols using h 600 transmission electron microscope
1
Ultrastructural Analysis of Ocular Tissues
2
Transmission Electron Microscopy of Retinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 13-LGSs were euthanized by decapitation without recovering from anesthesia, as described previously.26 Globes were immediately enucleated and fixed overnight at 4°C in 0.1 M cacodylate buffer containing 2% paraformaldehyde and 2% glutaraldehyde. The cornea and lens were then removed, the eye cups treated in 1% osmium buffer on ice, dehydrated using an increasing series of methanol concentrations (50%–100%), washed in acetonitrile; and embedded in 100% Epon. Embedded samples were sent to the Medical College of Wisconsin Electron Microscopy Core Facility for sectioning at a thickness of 70 nm using a PowerTome MT-XL ultramicrotome (RMC Boeckeler, Tucson, AZ, USA). Thin sections were placed on 200 mesh hexagonal grids (EMS, Hatfield, PA, USA), stained with lead citrate and uranyl acetate, and imaged with an H-600 transmission electron microscope (Hitachi High Technologies, Schaumburg, IL, USA). All images were obtained immediately inferior to the optic disc in the area of the visual streak, with eyes sectioned medially so that all samples included a portion of the horizonal optic disc, allowing us to maintain orientation.
3
Histological Analysis of Hippocampus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hippocampus tissues stained with HE were processed by standard procedures. The sections (5 μm) were observed and photographed with an Olympus AH-2 light microscope (Olympus, Tokyo, Japan). For TEM analysis, 1-mm3 tissue blocks of the hippocampus samples were placed in 3% glutaraldehyde solution with 0.1 M phosphate-buffered solution (PBS). Samples were post-fixed for 1.5 h in 1% osmium tetroxide solution, washed in 0.1 M PBS, dehydrated in graded concentrations of ethanol, and embedded in Epon 618. The epoxy blocks were sliced on an ultramicrotome (LKB-V, LKB, Stockholm, Sweden, 70 nm), stained with uranyl acetate and lead citrate, and examined using a Hitachi H600 transmission electron microscope (Hitachi, Co., Tokyo, Japan). Digital images were computationally acquired.
4
Internode Cell Wall Growth Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To characterize cell wall growth in different parts of internode 18 of P. edulis with a length of 14 cm, each 1-cm section was taken from the bottom to the top. The uppermost 1-cm section of a 23-cm-long internode 18 and the uppermost 1-cm sections of an internode 18 with a length of 29 cm at 0, 20, and 50 days after the rapid growth stage were also sampled. The green outer skins and yellow inner skins of these sections were removed, and the sections were immediately fixed in 2.5% glutaraldehyde for 20 h at 4°C. Samples were then sectioned and observed using an H-600 transmission electron microscope (Hitachi, Tokyo, Japan) as described by Wang et al. (2019) (link).
5
Ultrastructural Analysis of Mouse Eyes
6
Ultrastructural Analysis of SMMC-7721 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SMMC-7721 cells in each experimental group were collected, digested by 2.5g/L trypsin, centrifuged at 3000 r/min, washed by PBS and kept in EP tubes. Next, cells were fixed first by 25 g/L glutaraldehyde, then by 10g/L osmic acid, dehydrated by graded ethanol, infiltrated, and embedded in epoxy resin. After slicing by the ultramicrotome, cells were stained by uranyl acetate and lead citrate. Next, a Hitachi H-600 transmission electron microscope was used for observation, filming, and photographing.
7
Transmission Electron Microscopy of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The transmission electron microscope of the electron microscopy center of Ningxia Medical University was used. Before the cells were observed, they were fixed in phosphate-buffered 2.5% glutaraldehyde for 24 h, and then post-fixed in phosphate-buffered 2% osmium tetroxide for 2 h. Subsequently, the cells were dehydrated in ascending acetone concentrations, and then infiltrated with Spurr's resin for over 24 h. The cells were subsequently observed under a Hitachi H-600 transmission electron microscope (Ibaraki, Japan).
8
Characterization of Polymer Micelles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 1H NMR spectra was recorded on a Bruker AV II-400. The molecular weight distribution was measured by an Agilent 1260 gel permeation chromatography (GPC) using THF as the eluent (flow rate: 1 mL/min at 40 oC) and the calibration curve was obtained from a series of narrow PS standards. The size and zeta potential of micelles were measured by a Malvern Zetasizer Nano ZS at 25 oC. Transmission electron microscopy (TEM) images were obtained on a Hitachi H-600 transmission electron microscope. The AIE behavior was measured on a Hitachi fluorescence spectrophotometer F-4700.
9
Ultrastructural Changes in Chlorella vulgaris
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission Electron Microscopy (TEM) was used to observe the ultrastructural changes of Chlorella vulgaris. 2.5% glutaraldehyde, prepared in 0.1 M cacodylate buffer and post-fixed in 1% osmium tetroxide in the same buffer, was used to fix cells (control and treated samples). After dehydration with acetonitrile, the pellets were embedded in Epon. Later, a Leica UC7 ultramicrotome was used to cut thin section (90 nm) and collected on 150-mesh copper grids. Sections were observed with a Hitachi H-600 transmission electron microscope (Tokyo, Japan) at 75 kV, after staining with 2% uranyl acetate in 50% ethanol and incubation with a lead citrate solution.
10
Nanoparticle Visualization by Transmission Electron Microscopy
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!