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Male fvb mice

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Male FVB mice are a mouse strain commonly used in biomedical research. They are inbred laboratory mice that carry the FVB genetic background.

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Lab products found in correlation

Laboratory rodent diet 5001, by Land O'Lakes (2 mentions) Aluminum hydroxide alum, by Thermo Fisher Scientific (2 mentions) Chicken ovalbumin ova, by Avantor (2 mentions) Zymosan, by Merck Group (1 mentions) Proteome profiler mouse xl cytokine array kit, by R&D Systems (1 mentions)

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FVB mice (47 citations)

15 protocols using male fvb mice

1

Mouse Peritonitis Model for Inflammatory Response

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All animal procedures were approved by the Standing Committee on Animals of Harvard Medical School (Protocol 02570) and performed in accordance with institutional guidelines. Male FVB mice (6–8 weeks old, weighing 22–25 g) were purchased from Charles River Laboratories. Mice were housed 4 mice per cage in specific pathogen-free facilities in a humidity (45–55%) and temperature (23–25°C) controlled environment with a 12 h light-dark cycle. Mouse peritonitis was carried out as in [10] (link) and efforts were made to minimize suffering (e.g. if an increase in pain or stress behavior was noted, the mice were euthanized according to Protocol #02570). Briefly, mice were anesthetized by inhaled isofluorane according to Protocol 02570 and randomly assigned to be administered intravenously with 1 ng 13R,14S-diHDHA, MaR1, or vehicle (saline) immediately prior to the administration of 0.1 mg zymosan [11] . At 4 h, mice were euthanized with overdose of isoflurane followed by cervical dislocation and peritoneal lavages collected. PMN numbers were assessed by light microscopy and flow cytometry. PMNs were identified as CD11b+ and Ly6G+ cells.
Deng B., Wang C.W., Arnardottir H.H., Li Y., Cheng C.Y., Dalli J, & Serhan C.N. (2014). Maresin Biosynthesis and Identification of Maresin 2, a New Anti-Inflammatory and Pro-Resolving Mediator from Human Macrophages. PLoS ONE, 9(7), e102362.
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2

Zymosan-Induced Peritoneal Inflammation in Mice

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Male FVB mice (10–12 weeks old) purchased from Charles River Laboratories were fed ad libitum Laboratory Rodent Diet 20–5058 (Lab Diet, Purina Mills). All animal experimental procedures were approved by the Standing Committee on Animals of Harvard Medical School (protocol no. 02570) and complied with institutional and US National Institutes of Health (NIH) guidelines. Peritonitis: zymosan (1 mg/mL saline; Sigma-Aldrich) was injected intraperitoneally (i.p.) 15 min after intravenous (i.v.) administration of RvD3 (10 ng), or vehicle in 100 µL saline. Peritoneal lavages were collected 4 h after zymosan administration. Leukocyte numbers and differential counts were assessed using Turks solution and flow cytometry analysis as detailed below. Cytokine, chemokine, and enzyme levels were assessed in cell-free supernatants by multiplex membrane-based array analysis using the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems) using manufacturer’s instructions; levels were determined by chemiluminescence and densitometry using ImageJ. Lavages were also placed in two volumes of methanol and subjected to lipid mediator metabololipidomics.
Norris P.C., Arnardottir H., Sanger J.M., Fichtner D., Keyes G.S, & Serhan C.N. (2016). Resolvin D3 multi-level proresolving actions are host protective during infection. Prostaglandins, leukotrienes, and essential fatty acids, 138, 81-89.
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3

Investigating Macrophage Responses to n-3 DPA

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Male FVB mice (6 to 8 weeks old) purchased from Charles River Laboratories were fed ad libitum Laboratory Rodent Diet 20–5058 (Lab Diet, Purina Mills). All animal experimental procedures were approved by the Standing Committee on Animals of Harvard Medical School (protocol no. 02570) and complied with institutional and US National Institutes of Health (NIH) guidelines. Peritonitis was induced by zymosan injection (1 mg/mL) intraperitoneally (i. p.) and exudates were obtained 4 h later. Human macrophages were prepared from peripheral blood mononuclear cells following literature protocols.31 (link) Macrophages were suspended in DPBS+/+ (5 × 107 cells/mL) and incubated with n-3 DPA (1 μM) and 0.1 mg serum treated zymosan (StZ, 37 °C, 30 min, pH = 7.45). The incubations were stopped with 2 mL of ice-cold MeOH and mediators extracted over C18 columns as described above.
Aursnes M., Tungen J.E., Vik A., Colas R., Cheng C.Y., Dalli J., Serhan C.N, & Hansen T.V. (2014). Total Synthesis of the Lipid Mediator PD1n-3 DPA: Configurational Assignments and Anti-inflammatory and Pro-resolving Actions. Journal of Natural Products, 77(4), 910-916.
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4

Dietary Effects on FVB Mice

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Male FVB mice (6 to 8 weeks old) purchased from Charles River Laboratories were fed ad libitum Laboratory Rodent Diet 20-5058 (Lab Diet, Purina Mills). All animal experimental procedures were approved by the Standing Committee on Animals of Harvard Medical School (protocol no. 02570) and complied with institutional and US National Institutes of Health (NIH) guidelines.
Colas R.A., Dalli J., Chiang N., Vlasakov I., Sanger J.M., Riley I.R, & Serhan C.N. (2016). Identification and actions of the maresin 1 metabolome in infectious inflammation. Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452.
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5

Ethical Rodent Housing and Care

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Animals (mice) were housed and treated according to institutional guidelines of the University of Pittsburgh IACUC committee (Protocol 1105844). This follows guidelines established by NIH for ethical use of animals in biomedical research. The IACUC committee and the aforementioned protocol specifically approved this study.
Male Fisher 344 rats (2–3 months of age) were purchased from Harlan. Male FVB mice (2–3 months of age) were purchased from Charles River. Animals were allowed food and water ad libitum and handled according to the Ethics Statement above.
Bowen W.C., Michalopoulos A.W., Orr A., Ding M.Q., Stolz D.B, & Michalopoulos G.K. (2014). Development of a Chemically Defined Medium and Discovery of New Mitogenic Growth Factors for Mouse Hepatocytes: Mitogenic Effects of FGF1/2 and PDGF. PLoS ONE, 9(4), e95487.
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6

Cranial Defect Repair in Mice

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Male FVB mice (7-week old, Charles River Laboratories, Hollister, CA) were used for cranial defect surgery. Briefly, mice were anesthetized with 2.5% isoflurane, and after removing the overlying pericranium, 3.3-mm cranial defects were created on the right parietal bone using a trephine drill without damaging the underlying dura mater. 10 µL of rehydrated µRBs was injected and filled into the defect, 1 µL of thrombin (200 U/mL) was added to allow in situ crosslinking. These groups are designated as “inject”. To assess the effect of injection, the scaffolds fabricated using the sandwich method without passing through injection were included and designated as “implant”. To assess the effect of BMP-2, the injected µRBs containing 100 ng BMP-2 per scaffold were included and designated as “inject+BMP-2”. All scaffolds were made as acellular and ASC-laden groups. The mice with defect but no treatment were included as negative control, designated as “non-treated”.
Tang Y., Tong X., Conrad B, & Yang F. (2020). Injectable and in situ crosslinkable gelatin microribbon hydrogels for stem cell delivery and bone regeneration in vivo. Theranostics, 10(13), 6035-6047.
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7

Age and Strain Comparison in Mice

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Male BalbC mice, 2 months old and 20 months old, were obtained from The National Institute of Aging, and male FVB mice (6 to 8 weeks old) were from Charles River Laboratories. All mice were maintained in a temperature- and light-controlled environment and were fed standard diet (PicoLab Rodent Diet 20 - 5053; Lab Diet) and water ad libitum. Animal experiments were approved by the Standing Committee on Animals of Harvard Medical School (protocol 02570) and performed in accordance with institutional guidelines
Arnardottir H.H., Dalli J., Colas R.A., Shinohara M, & Serhan C.N. (2014). Aging delays resolution of acute inflammation in mice: reprogramming the host response with novel nanoproresolving medicines. Journal of immunology (Baltimore, Md. : 1950), 193(8), 4235-4244.
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8

Eicosanoid Profiling in Murine Peritonitis

Cited 1 time
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Male FVB mice (6 to 8 weeks old) purchased from Charles River Laboratories were fed ad libitum Laboratory Rodent Diet 20–5058 (Lab Diet, Purina Mills). Animal experimental procedures were approved by the Standing Committee on Animals of Harvard Medical School (protocol no. 02570) and complied with institutional and US National Institutes of Health (NIH) guidelines. Mice were given vehicle together with live E. coli (serotype O6:K2:H1; 107 CFU) or saline injections (mock infection) i.p. After 12 h, mice were euthanized and the peritoneal exudate was collected, see reference 29 (link) for details. The local eicosanoid levels and identification PTAD-products were assessed by targeted lipid mediator metabololipidomics as described below.
Hansen T.V., Dalli J, & Serhan C.N. (2016). Selective identification of specialized pro-resolving lipid mediators from their biosynthetic double di-oxygenation isomers. RSC advances, 6(34), 28820-28829.
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9

FVB Mouse Housing and Care

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Male FVB mice were purchased from Charles River Laboratory or Model Animal Research Center of Nanjing University. The animals were housed in a 12-hour light/dark schedule with free access to food and water. Animal use was in full compliance with the NIH guidelines and was approved by our institutional Animal Care and Use Committees.
Jin N., Yin X., Yu D., Cao M., Gong C.X., Iqbal K., Ding F., Gu X, & Liu F. (2015). Truncation and activation of GSK-3β by calpain I: a molecular mechanism links to tau hyperphosphorylation in Alzheimer's disease. Scientific Reports, 5, 8187.
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10

Analyzing Human Vagus Nerve Responses

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Fresh human vagus (de-identified) purchased from Tissue for Research (Ellingham, Bungay, Suffolk, UK) were analyzed under protocol #1999P0001279 approved by Partners Human Research Committee. Each post-mortem, full-length human vagus was thawed on arrival, measured, dissected, and incubated in PBS (with calcium and magnesium) 20 min, 37°C with 5% CO2, in parallel with direct electrical vagus stimulation (EVS) with 2.5 mA 18V DC, 20 min, in PBS, 37°C (ApeX Type A stimulator, ApeX Electronics LLC, Schenectady, NY), or coincubated with E. coli (109 c.f.u., 3h, 37°C). Deuterium-labeled standards for SPM and eicosanoid extraction recoveries were from Cayman Chemical (Ann Arbor, MI). For abbreviations and stereochemical assignments with full name for each SPM, see (11 (link), 12 (link)). Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Brigham and Women’s Hospital (protocol no. 2016N000145) and complied with institutional and U.S. NIH guidelines. Six- to eight-week-old FVB male mice (Charles River Laboratories, Wilmington, MA) were fed ad libitum Laboratory Rodent Diet 20–5058 (Purina Mills, Great Summit, MO).
Serhan C.N., De la Rosa X, & Jouvene C.C. (2018). Human Vagus Produces Specialized Pro-Resolving Mediators of Inflammation with Electrical Stimulation Reducing Pro-Inflammatory Eicosanoids. Journal of immunology (Baltimore, Md. : 1950), 201(11), 3161-3165.
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