The NMR spectra were measured with a BRUKER AVANCE-600 MHz and AVANCE-400 MHz NMR spectrometers (Bruker Corporation, Billerica, Massachusetts, USA) at 24 °C. The IR spectra were recorded with a Varian 640t FTIR spectrometer (Varian Medical Systems, Palo Alto, California, USA) with samples in KBr pellets. The mass spectra were obtained with a MALDI-TOF Reflex 3 instrument (BRUKER (Bruker Corporation, Billerica, Massachusetts, USA)) in the positive ion mode (UV laser, 337 nm), without use of matrix. All of the reagents and solvents were obtained from commercial sources. The Acetonitrile (99.95%, Biosolve BV (Biosolve Chemicals, Dieuze, France)) was dried over molecular sieves (zeolite KA, 3 Å, balls, diameter 1.6–2.5 mm) prior to use. The water content was estimated as 40 ± 5 ppm by Karl Fisher titration (Mettler Toledo, C20, coulometric KF titrator (Mettler-Toledo, Inc., Columbus, Ohio, USA). Lanthanide metal nitrates Ln(NO3)3·6H2O (n = 4–6, purity > 99%) were stored in a closed container over silica gel balls. The stock solutions of ligands and metal salts were prepared by weighing the amounts of the respective chemicals and dissolving them in Acetonitrile.
Avance 600 mhz
Manufactured by Bruker
Sourced in Germany, United States, Switzerland
The Avance 600 MHz is a high-performance nuclear magnetic resonance (NMR) spectrometer designed by Bruker. It operates at a frequency of 600 MHz and is capable of advanced NMR experiments for structural analysis and quantification of chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Topspin, by Bruker (4 mentions)
Topspin 3, by Bruker (4 mentions)
Avance 500 mhz, by Bruker (3 mentions)
Topspin 2, by Bruker (3 mentions)
Jy98 iiin, by Ningbo Xinzhi (3 mentions)
1260 infinity quaternary lc, by Agilent Technologies (2 mentions)
241 polarimeter, by PerkinElmer (2 mentions)
5 mm tci inverse detection cryoprobe, by Bruker (2 mentions)
Enspire multimode reader, by PerkinElmer (2 mentions)
Alamarblue reagent, by Bio-Rad (2 mentions)
Ifs 66 s, by Bruker (2 mentions)
Tci cryoprobe, by Bruker (2 mentions)
Drx 500 mhz, by Bruker (2 mentions)
Drx 400 mhz, by Bruker (2 mentions)
Lct premier micromass spectrometer, by Waters Corporation (2 mentions)
Avance 500, by Bruker (2 mentions)
2767 sample manager, by Waters Corporation (2 mentions)
3100 mass detector, by Waters Corporation (2 mentions)
Cryoprobe, by Bruker (2 mentions)
Avance 300 mhz, by Bruker (2 mentions)
124 protocols using avance 600 mhz
1
NMR, IR, and Mass Spectrometry Analysis
2
Characterization of Gold-Polymer Assemblies
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The structures of PCL, PMEO2MA and Au@PCL/PMEO2MA were examined by NMR (Bruker Avance 600 MHz, Bruker AXS Inc, Madison, Wisconsin). The morphology of the gold assemblies (GAs) was observed by transmission electron microscope (TEM, JEOL 2100F) at 200 kV and field emission scanning electron microscope (FESEM, JSM 6700F). Thermo gravimetric analysis (TGA) was obtained using a TG-209-F3 thermo gravimetric analyzer (Netzsch Instruments, Germany) with a heating rate of 10 °C/min from 30 to 700 °C in N2 atmosphere. The STEM and EDS measurements were carried out using field emission transmission electron microscopy (FETEM, Tecnai G2 F30). UV vis spectrophotometry measurements were performed on TU-1810 ultraviolet and visible spectrophotometer (Persee, China) with wavelength range from 400 nm to 1100 nm. ICP-MS measurement for gold materials were performed on inductively coupled plasma mass spectrometry (Agilent 7500ce). The photothermal images were obtained by using thermal camera (FLIR, T330) running on FLIR tools systems.
3
HPLC Analysis and Purification Protocol
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All chemical reagents and solvents were taken from commercial sources and used without purification. High-performance liquid chromatography analyses were carried out on a Waters Binary Module System (Waters, Milford, MA, USA) with a dual λ absorbance detector system fitted with a Supelco Ascentis C8 HPLC column (250 mm × 4.6 mm, 5 µm, Supelco, Bellefonte, PA, USA) with a 1 mL/min flow rate operating on a linear gradient from 5 to 95% B within 20 min (solvent A: H2O with 0.05% trifluoroacetic acid, solvent B: MeCN with 0.05% trifluoroacetic acid). High-performance liquid chromatography purification was carried out in a Varian ProStar system (Varian, Australia) with a dual λ absorbance detector system with the application of a Supelco Ascentis C8 HPLC column (250 mm × 21.2 mm, 5 µm) with a 15 mL/min flow rate and a linear gradient from 20 to 100% B within 20 min or 40 to 100% B within 20 min (depending on compound solubility), solvent A: H2O with 0.05% trifluoroacetic acid, solvent B: MeCN with 0.05% trifluoroacetic acid. The nuclear magnetic resonance spectra (1H and 31P) were recorded on a 400 MHz NMR Jeol PCZ 400S or Bruker Avance 600 MHz (Bruker, Billerica, MA, USA). High resolution mass spectra were recorded with a Brucker micro TOF-Q II (Bruker, Billerica, MA, USA).
4
Characterization of Organic Compounds
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Optical rotations were measured in CH>2Cl2 on a PerkinElmer 241 polarimeter (Waltham, MA, USA) by using a Na lamp. NMR spectra were recorded on a Bruker AVANCE 500 MHz or 600 MHz (Bruker Biospin, Falländen, Switzerland), as required. NMR spectra were obtained dissolving samples in CDCl3 (99.9%) and chemical shifts are reported relative to solvent (δH 7.26 and δC 77.0 ppm). Bruker AVANCE 600 MHz instrument is equipped with a 5 mm TCI inverse detection cryoprobe (Bruker Biospin, Falländen, Switzerland). Standard Bruker NMR pulse sequences were utilized. HR-ESI-MS data were obtained on an Waters LCT Premier XE Micromass (Manchester, UK) and VG -AutoSpec Micromass spectrometers (Manchester, UK), respectively. IR spectra were recorded on a Bruker IFS66/S (Ettlingen, Germany) equipped with an ATR accessory using CH2Cl2 solutions. EnSpire® Multimode Reader (Perkin Elmer, Waltham, MA, USA) using absorbance values of Alamar Blue® reagent (Bio-Rad Laboratories, Oxford, UK). HPLC (High performance liquid chromatography) separations were carried out with an Agilent 1260 Infinity Quaternary LC equipped with a Diode Array Detector (Waldbronn, Germany). TLC (Thin layer chromatography) (Merck, Darmstadt, Germany) was visualized by UV light (254 nm) and spraying with cobalt chloride reagent (2% in sulfuric acid, 10%) and heating.
5
NMR Spectroscopic Analysis of Lipid Components
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1H NMR
spectra were collected from undigested lipid components and lipids
extracted from digesta. For the analysis, 500 μL of sample was
dried with N2 flow and recollected to 200 μL of CDCl3/DMSO-d6 (5:1, v/v, previously
dried with 4 Å molecular sieves), of which 180 μL was pipetted
into 3 mm NMR tubes. Samples were prepared the previous day before
the analysis and stored overnight at −20 °C in desiccators. 1H NMR spectra were collected at 298 K with a Bruker Avance
600 MHz (Bruker Biospin, Switzerland) equipped with a Prodigy TCI
CryoProbe and SampleJet sample changer. Proton spectra were collected
with 32 scans, an acquisition time of 4 s, and a relaxation time of
5 s. A selective gradient excitation pulse program (selgpse) was applied for region-specific excitation of hydroperoxide (11.5–10.5
ppm) and aldehyde (10–9 ppm) protons. The program had 4 dummy
scans and 128 scans, with an acquisition time of 2.7 s and a relaxation
time of 5 s. The 180-degree shaped pulse had a length of 1566.15 s.12 (link) NMR data were processed with TopSpin 4.0.7 (Bruker,
MA, USA).
spectra were collected from undigested lipid components and lipids
extracted from digesta. For the analysis, 500 μL of sample was
dried with N2 flow and recollected to 200 μL of CDCl3/DMSO-d6 (5:1, v/v, previously
dried with 4 Å molecular sieves), of which 180 μL was pipetted
into 3 mm NMR tubes. Samples were prepared the previous day before
the analysis and stored overnight at −20 °C in desiccators. 1H NMR spectra were collected at 298 K with a Bruker Avance
600 MHz (Bruker Biospin, Switzerland) equipped with a Prodigy TCI
CryoProbe and SampleJet sample changer. Proton spectra were collected
with 32 scans, an acquisition time of 4 s, and a relaxation time of
5 s. A selective gradient excitation pulse program (selgpse) was applied for region-specific excitation of hydroperoxide (11.5–10.5
ppm) and aldehyde (10–9 ppm) protons. The program had 4 dummy
scans and 128 scans, with an acquisition time of 2.7 s and a relaxation
time of 5 s. The 180-degree shaped pulse had a length of 1566.15 s.12 (link) NMR data were processed with TopSpin 4.0.7 (Bruker,
MA, USA).
6
NMR Spectroscopy of PD-L1 Binding
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NMR spectra were recorded in PBS, pH 7.4, containing 10% (v/v) of D2O added to the samples to provide the lock signal. Water suppression was carried out using the WATERGATE sequence. All the spectra were recorded at 300 K using a Bruker Avance 600 MHz spectrometer with the Cryo-Platform. The NMR spectrum was recorded at three different ligand/protein ratios from 0 to 10. The samples were prepared by adding small amounts of a 50 mM ligand stock solution in DMSO to the protein solution (0.20 mL) of the PD-L1 fragment at a concentration of 0.14 mM. The acquisition parameters for each spectrum were as follows: size of FID32768, number of scans: 32. The spectra were visualized using TopSpin 4.0.2. (Bruker, Billerica, MA, USA)
7
Comprehensive Spectroscopic and Thermal Analysis
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1H, 13C and 29Si NMR spectra were recorded on a Bruker Avance 600 MHz nuclear magnetic resonance spectrometer using chloroform-d. A thermogravimetric analysis (TGA) was performed using a Thermowaage STA409. The analysis was measured in air, with a flow rate of 50 mL/min. The heating rate of 10 °C/min was used from room temperature to 900 °C. DSC were recorded on TA instruments DSC 2910 using standard DSC cell. SEM photographs were observed using JEOL JSM-7000F Field Emission Analytical Scanning Electron Microscopy.
Prior to N2 sorption measurements, all the samples were degassed at 100 °C under vacuum overnight. Nitrogen adsorption–desorption isotherms were recorded on a Quantochrome Nova 2000 instrument. The specific surface area was calculated using the multipoint Brunauer–Emmett–Teller (BET) method. Pore size distributions were calculated by the Barrett, Joyner and Halenda (BJH) method. Pore volumes were determined from the amount of N2 adsorbed at P/P0 = 0.99.
Prior to N2 sorption measurements, all the samples were degassed at 100 °C under vacuum overnight. Nitrogen adsorption–desorption isotherms were recorded on a Quantochrome Nova 2000 instrument. The specific surface area was calculated using the multipoint Brunauer–Emmett–Teller (BET) method. Pore size distributions were calculated by the Barrett, Joyner and Halenda (BJH) method. Pore volumes were determined from the amount of N2 adsorbed at P/P0 = 0.99.
8
NMR Spectroscopy of Polypeptide Backbone
9
NMR Characterization of Nucleic Acid Duplexes
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The tc-DNA•RNA, tc-DNA•DNA and tc-DNA•tc-DNA duplexes were prepared at 0.5 mM concentration in 50 mM NaCl and 10 mM NaH2PO4 buffer solution, pH 7.05. To prepare the samples, equimolar quantities of the complementary strands were combined and annealed by heating to 90°C and then slowly cooled to room temperature. NMR spectra were measured in either 99.990% D2O or 90% H2O/10% D2O on Bruker Avance 600 MHz spectrometer equipped with a 5 mm TCI CryoProbe™ and Bruker Avance 700 MHz equipped with a 5 mm TXI CryoProbe™. DQF-COSY, TOCSY (mixing time of 80 ms), 13C–1H HSQC, 13C–1H HMQC, 1H–31P HETCOR and NOESY (mixing time of 60, 100, 150 and 250 ms) spectra in D2O were recorded at 283 and 298 K. NOESY (mixing time of 250 ms) spectra in H2O were measured at 283 K in order to reduce exchange with water. All 2D spectra were processed by NMRPipe (23 (link)) and analyzed using SPARKY (24 ).
10
Synthesis and Characterization of Chiral Compounds
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Unless otherwise stated, reactions were performed under a nitrogen atmosphere using dried solvents. Commercially available reagents were used without further purification. Thin-layer chromatography (TLC) was performed using Jiangyou TLC silica gel plates HSG F254 and visualized using ultraviolet light or phosphomolybdic acid. Flash column chromatography was performed over silica gel (200 to 300 mesh). 1H and 13C nuclear magnetic resonance spectra were recorded in chloroform-d, unless otherwise noted, on a Bruker AVANCE 600-MHz or a Bruker AVANCE 400-MHz spectrometer. High-resolution electrospray ionization and electronic impact mass spectrometry was performed on a Thermo Scientific Q Exactive mass spectrometer (mass analyzer type: Orbitrap). Analytical chiral high-performance liquid chromatography (HPLC) was performed with an Agilent 1260 Series HPLC using CHIRALPAK AS-H (4.6 mm by 25 cm), CHIRALCEL AD-H (4.6 mm by 25 cm), CHIRALCEL OJ-H (4.6 mm by 25 cm), or CHIRALCEL OD-H columns (4.6 mm by 25 cm) obtained from Daicel Chemical Industries Ltd. with visualization at 254 or 210 nm.
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