The largest database of trusted experimental protocols

Enhanced chemiluminescence ecl kit

Manufactured by Sartorius

The enhanced chemiluminescence (ECL) kit is a laboratory product designed to detect and analyze protein expression levels. It utilizes a chemiluminescent reaction to generate a luminous signal that is proportional to the amount of target protein present in a sample. The kit provides the necessary reagents and solutions to perform this analysis.

Automatically generated - may contain errors

2 protocols using enhanced chemiluminescence ecl kit

1

Immunoblotting Antibodies and Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibody against LC3 (1:2,000; cat. no. NB100-2220) was purchased from Novus Biologicals, LLC. The antibody against sequestosome 1 (SQSTM1)/p62 (1:2,000; cat. no. ab56416) was purchased from Abcam. Antibodies against β-actin (1:1,000; cat. no. 60008-1-Ig), LAMP1 (1:100; cat. no. 21997-1-AP) and Beclin1 (1:1,000; cat. no. 11306-1-AP) were purchased from ProteinTech Group, Inc. Antibodies against cathepsin D (1:1,000; cat. no. sc-6486) and goat anti-rabbit immunoglobulin G (IgG)-fluorescein isothiocyanate (FITC) (1:100; cat. no. SC2-12) were purchased from Santa Cruz Biotechnology, Inc. Horseradish peroxidase (HRP)-conjugated anti-rabbit (1:10,000; cat. no. W4011), HRP-conjugated anti-goat (1:10,000; cat. no. V805A) and HRP-conjugated anti-mouse (cat. no. W4021) antibodies were purchased from Promega Corporation. Enhanced chemiluminescence (ECL) kit was purchased from Biological Industries.
+ Open protocol
+ Expand
2

Multiplex Immunohistochemistry for Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-TRPV1 (1:100, ab6166), anti-LC3 (1:1000, ab192890), anti-P62 (1:1000, ab109012), anti-LAMP2 (1:100, ab13524), anti-H2 Db/H2-D1(1:100, ab25244), and Alexa Fluor 568 (1:500), Alexa Fluor 488 (1:500) secondary antibody were purchased from Abcam. Anti-CGRP (1:100, sc-57053) was purchased from Santa cruze. Anti-mouse CD45-PE-cy7 (103114), anti-mouse CD11c-APC-cy7 (117323), anti-mouse CD3-APC-cy7 (100221), anti-mouse CD8a-APC (100711), anti-mouse CD16/32-TruStain FcX™ (101320), anti-mouse CD86-APC (105011) antibodies were purchased from BioLegend. APC anti-mouse H-2 (E-AB-F1216E) and Anti-mouse CD80-FITC (E-AB-F0992C) were purchased from Elabscience. HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were purchased from Promega. Enhanced chemiluminescence (ECL) kit was purchased from Biological Industries. One Step TUNEL Apoptosis Assay Kit (Green Fluorescence) (C1088) and Ad-GFP-LC3B (C3006) were purchased from Beyotime. Lyso-Tracker Red (L12492) was purchased from Invitrogen. Imiquimod (HY-B0180A) was purchased from MCE. Indocyanine Green (3599-32-4) was purchased from Aladdin. Morphine (161007-2) was purchased from NORTHEAST PHARM. Ropivacaine (R413090) was purchased from Mackline. Pluronic F127 (P2443) was purchased from Sigma.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!